亚麻<em>MS2</em>-<em>F</em>基因的RNAi表达载体构建

doi:10.3969/j.issn.1000-7091.2012.03.003

华北农学报 ›› 2012, Vol. 27 ›› Issue (3): 12-16. doi: 10.3969/j.issn.1000-7091.2012.03.003

所属专题： 生物技术；

亚麻MS2-F基因的RNAi表达载体构建

斯钦巴特尔, 张辉, 贾霄云, 高风云, 张立华, 任龙梅

蒙古农牧业科学院胡麻课题组, 内蒙古呼和浩特 010031

收稿日期:2012-02-15 出版日期:2012-06-28
通讯作者: 张辉（1961-）, 女, 内蒙古乌兰察布人, 研究员, 主要从事亚麻遗传育种与植
作者简介:斯钦巴特尔（1965-）, 男, 内蒙古赤峰人, 副研究员, 博士, 主要从事植物分子生物学及基因工程研究。
基金资助:
国家自然科学基金(30860148;31060197)

Construction of RNAi Vectors of Flax Male Sterility Related MS2-F Gene

SIQIN Bateer, ZHANG Hui, JIA Xiao-yun, GAO Feng-yun, ZHANG Li-hua, REN Long-mei

Flax Research Group, Inner Mongolia Academy of Agricultural and Husbandry Science, Hohhot 010031, China

Received:2012-02-15 Published:2012-06-28

摘要/Abstract

摘要： 亚麻雄性不育相关基因MS2-F在亚麻花粉发育过程中起重要的作用。为了研究其功能,构建了2个RNAi载体。选定该基因的2个不同的片段,分别以正向和反向插入到pHANNIBAL载体中PDK intron的两侧,以形成hpR-NA表达盒。然后将hpRNA表达盒插入到pART27的NotⅠ酶切位点上,成功构建了2个亚麻MS2-F基因的RNAi载体。最后将载体导入到农杆菌LBA4404中。RNAi载体成功构建,为验证MS2-F基因在亚麻花粉发育过程中的生物学功能奠定基础。

Abstract: Flax male sterility related MS2-F gene plays an important role in the process of flax pollen development. In order to conform the function of this gene，two RNAi vectors of the gene were constructed. Two different?fragments were selected and inserted into pHANNIBAL flanking PDK intron in sense orientation and antisense orientation，thus forming hpRNA cassettes. Then the Not I fragment from pHANNIBAL containing the hpRNA cassettes?were subcloned into the binary vector pART27. Finally the vectors were introduced into Agrobacterium LBA4404.?These successfully constructed RNAi vectors and subsequent genetic transformation will provide useful clues for revealing the function of MS2-F gene during pollen development in flax.

Key words: Fax, Male sterility related MS2-F gene, Function conformation, RNAi vector

中图分类号:

S563.2

斯钦巴特尔, 张辉, 贾霄云, 高风云, 张立华, 任龙梅. 亚麻MS2-F基因的RNAi表达载体构建[J]. 华北农学报, 2012, 27(3): 12-16. doi: 10.3969/j.issn.1000-7091.2012.03.003.

SIQIN Bateer, ZHANG Hui, JIA Xiao-yun, GAO Feng-yun, ZHANG Li-hua, REN Long-mei. Construction of RNAi Vectors of Flax Male Sterility Related MS2-F Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 12-16. doi: 10.3969/j.issn.1000-7091.2012.03.003.

http://www.hbnxb.net/CN/Y2012/V27/I3/12

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亚麻MS2-F基因的RNAi表达载体构建

Construction of RNAi Vectors of Flax Male Sterility Related MS2-F Gene

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