玉米ZmPT6基因的克隆及RNAi表达载体的构建

doi:10.7668/hbnxb.2009.05.003

华北农学报 ›› 2009, Vol. 24 ›› Issue (5): 11-15. doi: 10.7668/hbnxb.2009.05.003

所属专题： 玉米；生物技术；

玉米ZmPT6基因的克隆及RNAi表达载体的构建

朱先灿^1,2, 宋凤斌¹

1. 中国科学院东北地理与农业生态研究所, 吉林长春 130012;
2. 中国科学院研究生院, 北京 100049

收稿日期:2009-07-10 出版日期:2009-10-28
通讯作者: 宋凤斌(1963-)，男，吉林前郭人，博士生导师，研究员，主要从事作物生理生态学研究。
作者简介:朱先灿(1983-)，男，安徽太湖人，在读博士，主要从事作物生理与分子生态学研究。
基金资助:
国家高技术研究发展计划“863”资助项目(2006AA10Z227)

Cloning of Maize ZmPT6 Gene and Construction of RNAi Expression Vector

ZHU Xian-can^1,2, SONG Feng-bin¹

1. Northeast Institute of Geography and Agroecology, Chinese Academy of Science, Changchun 130012, China;
2. Graduate University of Chinese Academy of Science, Beijing 100049, China

Received:2009-07-10 Published:2009-10-28

摘要/Abstract

摘要： 采用PCR方法分别从玉米郑单958基因组DNA及RNA中克隆了菌根共生磷酸盐转运蛋白基因ZmPT6，序列分析表明，ZmPT6基因全长cDNA1665bp，包括一个长97bp的内含子；编码554个氨基酸，属于Pht1家族成员，由12个疏水的跨膜结构域组成。并构建了35S启动子驱动的植物正义、反义及RNAi表达载体，为下一步玉米的遗传转化及ZmPT6基因的功能研究奠定了基础。

关键词: ZmPT6基因, 正义表达载体, 反义表达载体, RNAi载体

Abstract: The arbuscular mycorrhizal symbiotic phosphate transporter gene ZmPT6 was cloned from Zea mays Zhengdan 958 genomic DNA and RNA by PCR method.Sequences analysis showed that ZmPT6 fulllength cDNA 1665bp,and contain a intron of 97bp.ZmPT6 belong to member of Pht1 family,encoded 554 amino acid,and consist of 12 putative hydrophobic transmembrane domains.Three plant transformation vectors,sense vector p2300-35S-ZmPT6,antisense p2300-35S-ZmPT6 r,and RNAi vector p2300-35S-ZmPT6 RNAi were constructed and so as to provide basis of further genetic transformation and function of ZmPT6 gene.

Key words: ZmPT6 gene, Sense expression vector, Antisense expression vector, RNAi vector

中图分类号:

Q785

朱先灿, 宋凤斌. 玉米ZmPT6基因的克隆及RNAi表达载体的构建[J]. 华北农学报, 2009, 24(5): 11-15. doi: 10.7668/hbnxb.2009.05.003.

ZHU Xian-can, SONG Feng-bin. Cloning of Maize ZmPT6 Gene and Construction of RNAi Expression Vector[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(5): 11-15. doi: 10.7668/hbnxb.2009.05.003.

http://www.hbnxb.net/CN/Y2009/V24/I5/11

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