摘要： 比较了卵母细胞级别对体外成熟率的影响,以及促卵泡素(FSH)处理山羊对卵母细胞采集的数量和质量的影响。结果表明:卵母细胞级别对体外成熟率有显著影响,不论是来源于FSH处理卵巢还是屠宰场卵巢,均是A、B级卵母细胞的成熟率高于C级卵母细胞,差异极显著(P<0.01)。但是A、B级卵母细胞之间差异不显著;卵巢来源对卵母细胞采集的数量和质量有显著影响,A、B级卵母细胞在获卵总数中所占的比例,平均每个卵巢获卵母细胞数及A、B级卵母细胞数,均是FSH处理卵巢高于屠宰场卵巢,差异显著(P<0.05);卵巢来源对卵母细胞体外成熟率有显著影响,来自于FSH处理卵巢的卵母细胞体外成熟率高于屠宰场卵巢(81.6%比67.6%),差异极显著(P<0.01)。
关键词:
卵母细胞,
分级,
体外成熟,
FSH处理,
山羊
Abstract: The aim of the present study was to compare the effects of oocytes grade on the rate of in vitro maturation，and the effect of the FSH-primed follicles on the numbers and quality of collecting oocytes． The result showed that:Oocytes grade had significant effects on the rate of in vitro maturation，grade A and B oocytes showed significantly higher percentage of maturation than that of Grade C，whatever the oocytes from the FSH-primed goats or from?slaughthouse ovaries(P＜0.01)． There was no signficent difference between Grade A and B oocytes． Ovaries source?had significant effects on the numbers and quality of collecting oocytes，FSH-primed ovaries was higher than slaughthouse ovaries，whatever the the percentage of Grade A and B oocytes in the total ones(P＜0.05)，the numbers of?total oocytes(P＜ 0.05) and Grade A and B oocytes (P＜ 0.05) from each ovary． Ovaries source had significant?effects on percentage of maturation，oocytes recovered from the FSH-primed goats showed significantly higher percentage of maturation than that of oocytes from slaughthouse ovaries(P＜0.01)．
Key words:
Oocytes,
Classification,
In vitro maturation,
FSH-primed,
Goats
中图分类号:
刘海军, 马群, 王儒, 郑梓, 张文昌. 来源和质量不同对山羊卵母细胞体外成熟率的影响[J]. 华北农学报, 2012, 27(2): 117-120. doi: 10.3969/j.issn.1000-7091.2012.02.023.
LIU Hai-jun, MA Qun, WANG Ru, ZHENG Zi, ZHANG Wen-chang. Effect of Source and Quality of Goat Oocytes on in vitro Maturation Rate[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(2): 117-120. doi: 10.3969/j.issn.1000-7091.2012.02.023.