华北农学报 ›› 2012, Vol. 27 ›› Issue (1): 189-192. doi: 10.3969/j.issn.1000-7091.2012.01.036

所属专题: 小麦

• 论文 • 上一篇    下一篇

河南两地市小麦白粉病菌的分子鉴定和进化分析

张怡1, 张佩佩2, 马晓萌1, 傅露露1, 乔月娥1, 陈佩佩1, 李成伟1   

  1. 1. 周口师范学院生命科学系, 植物遗传与分子育种重点实验室, 河南周口 466001;
    2. 河南师范大学生命科学学院, 河南新乡 453007
  • 收稿日期:2011-11-14 出版日期:2012-02-28
  • 通讯作者: 李成伟(1972- ), 男, 河南商丘人, 教授, 博士, 主要从事植物与病原体互作、抗性分子育种及生物反应器制药等研究。
  • 作者简介:张怡(1985- ), 女, 河南洛阳人, 助教, 硕士, 主要从事植物与微生物互作研究。
  • 基金资助:
    国家自然科学基金(31071807);周口师范学院青年科研基金项目(zknuqn201046B);周口师范学院实验室开放项目(201113)

Identification and Phylogenic Analysis of Powdery Mildew on Triticum aestivum in Two Cities of Henan

ZHANG Yi1, ZHANG Pei-pei2, MA Xiao-meng1, FU Lu-lu1, QIAO Yue-e1, CHEN Pei-pei1, LI Cheng-wei1   

  1. 1. Key Lab of Plant Genetics and Molecular Breeding, Department of Life Science of Zhoukou Normal University, Zhoukou 466001, China;
    2. Department of Life Science, Henan Normal University, Xinxiang 453007, China
  • Received:2011-11-14 Published:2012-02-28

摘要: 通过对河南省周口市和商丘市小麦白粉病菌进行分子鉴定及分析,以期为小麦白粉病菌的防治和系统进化奠定基础。对小麦白粉病菌进行了扫描电镜的显微形态观察、核糖体DNA转录间隔区(ITS) 序列测定及进化树分析。结果显示:周口和商丘的小麦白粉病菌均属于禾本科布氏白粉菌;系统进化发现,商丘的小麦白粉病菌与地域相距较远的英国、法国、美国的小麦白粉病菌的同源性极高,而与地域很近的周口小麦白粉病菌同源性却不高,推测小麦白粉病菌的进化可能与其自身的小种进化有关。

关键词: 小麦白粉病菌, 系统进化, 扫描电镜, DNA转录间隔区, 进化树

Abstract: Large of powder mildew was found on leves of Triticum aestivum in Zhoukou and Shangqiu of Henan province. In order to lay the foundation for powder mildew preventing, controlling and the system evolution, the characteristics of histological morphological with scanning electron microscope, internal transcribed spacer(ITS) region and phylogenetic trees were conducted. The results demonstrated that the wheat powdery mildew belongs to Blumeria graminis f. sp. tritici. While phylogenetic tree revealed the pathogen of Shangqiu was highly conserved with the UK, France and USA, which were widely far away from Shangqiu. However the wheat powdery mildew in Shangqiu and Zhoukou share low homology, we suggest that the evolution of B. graminis may be related to its own physiological race.

Key words: Blumeria graminis f. sp. tritici, System evolution, Scanning electron microscope, Internal transcribed spacer(ITS), Phylogenetic tree

中图分类号: 

引用本文

张怡, 张佩佩, 马晓萌, 傅露露, 乔月娥, 陈佩佩, 李成伟. 河南两地市小麦白粉病菌的分子鉴定和进化分析[J]. 华北农学报, 2012, 27(1): 189-192. doi: 10.3969/j.issn.1000-7091.2012.01.036.

ZHANG Yi, ZHANG Pei-pei, MA Xiao-meng, FU Lu-lu, QIAO Yue-e, CHEN Pei-pei, LI Cheng-wei. Identification and Phylogenic Analysis of Powdery Mildew on Triticum aestivum in Two Cities of Henan[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(1): 189-192. doi: 10.3969/j.issn.1000-7091.2012.01.036.

使用本文