华北农学报 ›› 2004, Vol. 19 ›› Issue (4): 18-20. doi: 10.3321/j.issn:1000-7091.2004.04.006

所属专题: 油料作物

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甘蓝型油菜辐照新不育系Mx1与Mx2及其保持系线粒体DNA的RAPD分析

林宝刚, 张明龙   

  1. 浙江大学原子核农业科学研究所, 浙江 杭州 310029
  • 收稿日期:2004-08-25 出版日期:2004-12-28
  • 作者简介:林宝刚(1979-),男,山东惠民人,硕士,主要从事油菜遗传育种的研究;张明龙为通讯作者.
  • 基金资助:
    浙江省科委重点攻关课题"杂交油菜"项目(G20010280)

RAPD Analysis on Mitochondrial DNA of the Cytoplasmic Male Sterile Lines Mx1 and Mx2 Induced by Radiation and Its Maintainer

LIN Bao-gang, ZHANG Ming-long   

  1. Institute of Nuclear Agricultural Science, Zhejiang University, Hangzhou310029, China
  • Received:2004-08-25 Published:2004-12-28

摘要: Mx1和Mx2是通过辐射甘蓝型油菜瑞典品种得到的细胞质雄性不育系。采用RAPD技术对Mx1和Mx2及其保持系陕2B的线粒体DNA(mtDNA)进行了比较研究。共使用了210个随机引物,其中有163个引物在两系之间都得到了扩增产物,15个引物扩增结果在两系之间表现出了遗传多态性。Mx1和Mx2间也存在着明显的差异。

关键词: 甘蓝型油菜, 辐射, 雄性不育系, mtDNA, RAPD分析

Abstract: Mx1 and Mx2 were two cytoplasmic male sterile lines which was obtained from Swedish variety of Brassica napus irradiated by gamma ray. A comparative analysis for mitochondrial DNAs ( mtDNAs) be- tween Mx1 and Mx2 and their maintainer were performed by using RAPD techniques. There were 163 primers giving amplified products in two lines. Among them 15 primers presented polymorphism. There was significant difference between Mx1 and Mx2 in RAPD amplified fragments.

Key words: Brassica napus L., Radiation, Cytoplasmic male sterile, Mitochondrial DNA, RAPD

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引用本文

林宝刚, 张明龙. 甘蓝型油菜辐照新不育系Mx1与Mx2及其保持系线粒体DNA的RAPD分析[J]. 华北农学报, 2004, 19(4): 18-20. doi: 10.3321/j.issn:1000-7091.2004.04.006.

LIN Bao-gang, ZHANG Ming-long. RAPD Analysis on Mitochondrial DNA of the Cytoplasmic Male Sterile Lines Mx1 and Mx2 Induced by Radiation and Its Maintainer[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2004, 19(4): 18-20. doi: 10.3321/j.issn:1000-7091.2004.04.006.

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