华北农学报 ›› 2004, Vol. 19 ›› Issue (1): 54-56. doi: 10.3321/j.issn:1000-7091.2004.01.015

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甜菜细胞质雄性不育系及保持系叶绿体DNA的RAPD分析

田自华1, 史树德2, 张剑峰3, 张子义1, 邵金旺1   

  1. 1. 内蒙古农业大学甜菜生理研究所, 内蒙古, 呼和浩特, 010018;
    2. 浙江大学, 生命科学学院, 浙江, 杭州, 310029;
    3. 内蒙古甜菜制糖工业研究所, 内蒙古, 呼和浩特, 010070
  • 收稿日期:2003-05-26 出版日期:2004-03-28
  • 作者简介:田自华(1960- ),男,内蒙古人,教授,硕士生导师,主要从事作物生理及生理育种科研与教学工作。
  • 基金资助:
    内蒙古自治区“人才专项基金”;内蒙古自然科学基金资助项目(20010903-04)

RAPD Analysis of Chloroplast DNA of Cytoplasmic Male Sterile Line and It's Maintainer Line in Sugarbeet

TIAN Zi-hua1, SHI Shu-de2, ZHANG Jian-feng3, ZHANG Zi-yi1, SHAO Jin-wang1   

  1. 1. Sugarbeet Physiological Institute, Inner Mongolia Agricultural University, Huhhot010018, China;
    2. College of Life Sciences, Zhejiang University, Hangzhou310029, China;
    3. Sugarbeet and Sugar Industry Institute of Inner Mongolia, Huhhot010070, China
  • Received:2003-05-26 Published:2004-03-28

摘要: 对细胞质雄性不育甜菜的不育系及其保持系的叶绿体DNA(ctDNA)进行了RAPD分析,结果表明:在所选的70个引物中,甜菜细胞质雄性不育系和保持系叶绿体DNA之间不存在差异性。与此同时,对叶绿体DNA的提取方法进行了改进,在不需密度梯度离心条件下即可获得高纯度的DNA;优化了甜菜叶绿体的RAPD的反应条件。

关键词: 甜菜, 细胞质雄性不育, 叶绿体DNA, RAPD

Abstract: Chloroplast DNA( ctDNA) come from cytoplasmic male sterile( CMS) it s maim ainer lineof sugarbeet was studied by means of rlom amplied polymorphic DNAs( RAPD).The fingerprints of the~plification by polyme chain reaction(PCR) of random DNA segments was enelysised.

Key words: RAPD, CMS, Sugerbeet, Choroplast DNA

中图分类号: 

引用本文

田自华, 史树德, 张剑峰, 张子义, 邵金旺. 甜菜细胞质雄性不育系及保持系叶绿体DNA的RAPD分析[J]. 华北农学报, 2004, 19(1): 54-56. doi: 10.3321/j.issn:1000-7091.2004.01.015.

TIAN Zi-hua, SHI Shu-de, ZHANG Jian-feng, ZHANG Zi-yi, SHAO Jin-wang. RAPD Analysis of Chloroplast DNA of Cytoplasmic Male Sterile Line and It's Maintainer Line in Sugarbeet[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2004, 19(1): 54-56. doi: 10.3321/j.issn:1000-7091.2004.01.015.

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