华北农学报 ›› 2003, Vol. 18 ›› Issue (3): 50-52. doi: 10.3321/j.issn:1000-7091.2003.03.013

所属专题: 黄瓜

• 论文 • 上一篇    下一篇

不同来源黄瓜遗传亲缘关系的RAPD分析

刘殿林1, 杨瑞环1, 哈玉洁1, 陈德富2, 陈喜文2, 夏立新2   

  1. 1. 天津市黄瓜研究所, 天津, 300192;
    2. 南开大学, 生物化学与分子生物学系, 天津, 300071
  • 收稿日期:2003-02-15 出版日期:2003-09-28
  • 作者简介:刘殿林(1971-),男,天津人,硕士,助理研究员学士,主要从事农业分子生物学的研究工作.
  • 基金资助:
    天津市重点攻关项目(993122511-2)

RAPD Analysis and Taxology Discussion on Cucumber Materials from Different Fountainhead Areas

LIU Dian-lin1, YANG Rui-huan1, HA Yu-jie1, CHEN De-fu2, CHEN Xi-wen2, XIA Li-xin2   

  1. 1. Tianjin Cucumber Research Institute, Tianjin 300192, China;
    2. Department of Biochemistry and Molecular Biology, Nankai University, Tianjin 300070, China
  • Received:2003-02-15 Published:2003-09-28

摘要: 用RAPD技术对黄瓜基因组DNA进行多态性研究,分析了9份黄瓜材料的遗传差异.结果表明,所采用的172条随机引物中,有49条引物扩增谱带清晰且重复性较好,扩增总片段数达78个,单个引物的扩增片段数在4~12之间,片段大小在0.2~.5 kb之间.不同材料间的遗传距离(D)在0.064~0.592之间,并根据遗传距离,按UWPGA法进行了聚类分析.

关键词: 黄瓜, RAPD, 遗传多样性, 聚类分析

Abstract: Random amplified Polymorphic DNA(RAPD) was used to analyze the genetic diversity among cucumber inbred line. The genetic of 39 cucumber inbred line was studied. The result showed that 49 of 172 arbitrary primers could amplify clear and repeatable bands. 398 fragments were obtained. Each primer gave 4-12 bands. The length of the band was 0.2-3.5 kb. The genetic distance(D) value between different breeds of cucumber inbred line was 0.064-0.592. The D value was used to construct a dendrogram by UPGMA.

Key words: Cucumber, RAPD, Genetic diversity, Cluster analysis

中图分类号: 

引用本文

刘殿林, 杨瑞环, 哈玉洁, 陈德富, 陈喜文, 夏立新. 不同来源黄瓜遗传亲缘关系的RAPD分析[J]. 华北农学报, 2003, 18(3): 50-52. doi: 10.3321/j.issn:1000-7091.2003.03.013.

LIU Dian-lin, YANG Rui-huan, HA Yu-jie, CHEN De-fu, CHEN Xi-wen, XIA Li-xin. RAPD Analysis and Taxology Discussion on Cucumber Materials from Different Fountainhead Areas[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2003, 18(3): 50-52. doi: 10.3321/j.issn:1000-7091.2003.03.013.

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