摘要： 以玉米愈伤组织为受体，借助GUS基因的短暂表达，优化了PDS-1000/He型基因枪和自制基因枪的基因枪转化参数。PDS-1000/He型基因枪转化玉米愈伤组织的最佳轰击参数为1350psi压力，25 In·Hg真空度，6cm射击距离。自制基因枪转化玉米愈伤组织的最佳轰击参数为0.6 MPa，10cm射击距离。
关键词:
玉米愈伤组织,
基因枪,
GUS基因短暂表达率,
GUS基因短暂表达单位
Abstract: Using maize calli as recipient cell,transforming parameters of PDS 1000/He gene delivery system and self made gene gun have been studied through GUS transient expression.The most suitable delivery parameters for PDS 1000/He gene delivery system are 1350psi of pressure,25 In·Hg of vacuum degree and 6 cm shoot distance of microcarrier;and for self made gene gun are 0.6 MPa pressure,10 c shoot distance of microcarrier.
Key words:
Maize calli,
Gene gun,
GUS transient ex pression rate,
GUS transient expression unit
中图分类号:
贾炜珑, 杨丽莉, 周小梅, 王景雪, 孙毅. 基因枪转化玉米愈伤组织参数的优化[J]. 华北农学报, 2001, 16(4): 77-80. doi: 10.3321/j.issn:1000-7091.2001.04.016.
JIA Weilong, YANG Lili, ZHOU Xiaomei, WANG Jingxue, SUN Yi. Optimization of Parameters Influencing Gene Delivery into Maize Calli by Gene Delivery System[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2001, 16(4): 77-80. doi: 10.3321/j.issn:1000-7091.2001.04.016.