摘要： 鲁棉6号自花授粉24h后,利用微注射技术,将多酚和单宁含量较高的罗布麻DNA导入其子房中。在高选择压力下,利用自然虫源,选育出了兼抗棉铃虫和棉蚜的棉花种质系115。对115和亲本鲁棉6号进行了室内生物学测定和食物利用试验检测,结果表明,抗虫种质系115严重阻碍了棉铃虫的食物利用,显著降低了初孵棉铃虫的存活、生长和发育。而鲁棉6号对棉铃虫影响较小。115幼叶棉酚和单宁的含量分别比鲁棉6号增加了90.9%和136.7%。
关键词:
棉花,
罗布麻,
DNA,
转育,
抗虫性
Abstract: After self-pollination twenty four hours,the exogenous bagbane(A.Venetum L.)DNA was introduced into ovaries of variety lumian 6(G.hirsutum)by injection method.In high selection stress,the second generation was screened for insect resistant by using natural insect.The stock 115 with resistance to bollworm and aphid was selected.Laboratory bioassay and food utilization of bollworm were used to test 115 and lumian 6.The results showed that leaf square and small boll of 115 highly inhibited food utilization of cotton bollworm,and significantly reduced larval surial,growth and development,whereas the effect was small in lumian 6.Contents of gossypol and tani in leaf of 115 were 90.9% and 136.7% as much as that of Lumian 6.
Key words:
Cotton,
Dogbane,
DNA,
Gene transfer,
Insect resistant
中图分类号:
沈法富, 于元杰, 张学坤, 刘风珍, 尹承佾. 罗布麻DNA导入棉花选育抗虫种质系[J]. 华北农学报, 1999, 14(4): 34-38. doi: 10.3321/j.issn:1000-7091.1999.04.007.
Shen Fafu, Yu Yuanjie, Zhang Xuekun, Liu Fengzhen, Yin Chengyi. Insect Resistant Stock Selected by Introducing Dogbane DNA into Cotton[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1999, 14(4): 34-38. doi: 10.3321/j.issn:1000-7091.1999.04.007.