华北农学报 ›› 1998, Vol. 13 ›› Issue (4): 36-41. doi: 10.3321/j.issn:1000-7091.1998.04.007

所属专题: 玉米 高影响力文章

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采用RAPD分子标记、表型和杂种优势聚类分析法对玉米自交系类群的划分

刘新芝, 彭泽斌, 傅骏骅, 黄长玲, 李连城   

  1. 中国农业科学院作物育种栽培研究所, 北京 100081
  • 收稿日期:1997-03-17 出版日期:1998-12-28
  • 基金资助:
    国家攀登计划资助

Compared Study of Molecular Marker with Two Normal Methods for Maize Inbred Lines Grouping

Liu Xinzhi, Peng Zebin, Fu Junhua, Huang Changling, Li Liancheng   

  1. Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences, Beijing 100081
  • Received:1997-03-17 Published:1998-12-28

摘要: 以15个我国生产上常用的玉米自交系为试验材料,采用表型性状、杂种优势和RAPD分子标记三种聚类分析方法对玉米自交系的组群划分进行了比较研究。结果表明,表型聚类因以表型性状为依据,受环境影响较大,其聚类结果与实际系谱来源出入较大。与表型性状聚类相比,杂种优势聚类与已知系谱来源更接近。RAPD分子标记,具有标记性状数量较多,不受生长发育时期限制和环境影响,无功能性表型效应,无上位及多效作用等诸多优点,其聚类结果与已知系谱符合率较高,反映出的类群关系比较可靠和真实。

关键词: 玉米自交系, 表型性状, 杂种优势, RAPD, 聚类分析

Abstract: Three methods of cluster analysis based on phenotype characters,heterosis,and RAPD's molecular markers,were compared in terms of methodology of heterosis grouping with 15 inbred lines used dominantly in commercial hybrid maize production in China.The analysis of phenotype cluster influenced by environment remarkably did not fit with the pedigree of the 15 entries.The heterosis cluster fitted better with the pedigree.RAPD's cluster fitted best with the pedigree for this method is based on plenty of molecular markers without functional phenotypic and multiple effects and epistasis,which are not influenced by growing and development stages and environment.RAPD's cluster is a reliable approach to distinguish the heterosis groups for inbred lines.

Key words: Inbred line of maize, Phenotype chatacter, Heterosis, RAPD, Cluster

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引用本文

刘新芝, 彭泽斌, 傅骏骅, 黄长玲, 李连城. 采用RAPD分子标记、表型和杂种优势聚类分析法对玉米自交系类群的划分[J]. 华北农学报, 1998, 13(4): 36-41. doi: 10.3321/j.issn:1000-7091.1998.04.007.

Liu Xinzhi, Peng Zebin, Fu Junhua, Huang Changling, Li Liancheng. Compared Study of Molecular Marker with Two Normal Methods for Maize Inbred Lines Grouping[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1998, 13(4): 36-41. doi: 10.3321/j.issn:1000-7091.1998.04.007.