华北农学报 ›› 1991, Vol. 6 ›› Issue (3): 61-64. doi: 10.3321/j.issn:1000-7091.1991.03.011

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鉴定染色体的新型染色剂与复合染色制片新技术

杨佩茹, 王瑞环, 张君明, 张淑娥   

  1. 河北省农林科学院蔬菜研究所, 石家庄 050051
  • 收稿日期:1990-09-15 出版日期:1991-09-28

A New Technology of Compound Stain Squash and a New Stain for Identification of Chromosomes

YangPeiru, WangRuihuan, ZhangJunming, ZhangShu'e   

  1. Vegetable Research Institute, Hebei Academy of Agricultural and ForestrySciences, Shijiazhuang
  • Received:1990-09-15 Published:1991-09-28

摘要: 选用黄瓜、茄子、莴苣、白菜、洋葱、萝卜6种蔬菜作物,以其幼叶为材料,采用新型染色剂PIHCH(丙酸-铁-苏木精-水合三氯乙醛),经单染或PIHCH和PICCH(丙酸-铁-洋红-水合三氯乙醛)复合染色制片,可清晰地观察到用一般染色剂很难着色的蔬菜作物叶片染色体.研究结果表明.这种新型染色剂和复合染色方法可以广泛应用于细胞学研究.

关键词: 染色剂, 蔬菜幼叶, 染色体, 复合染色

Abstract: They young leaves of cucumber, eggplant, lettuce, Chinese cabbage, Chinese radish and onion are made into squashes and then stained with a new single stain PIHCH (propionic-iron-haematohylin-chloral hydrate) and a compound stain of PIHCH and PICCH (propionic-iron-camine-chloral hydrate). The chromosomes which are hardly stained with common stains can be clearly observed under the microscops. Therefore the single stain PIHCH and the compound stain of PIHCH and PICCH can be widespreadly applyed in cytology study.

Key words: Stain PICCH, Young leaves of vegetable, Chromosomes, Compound staining

引用本文

杨佩茹, 王瑞环, 张君明, 张淑娥. 鉴定染色体的新型染色剂与复合染色制片新技术[J]. 华北农学报, 1991, 6(3): 61-64. doi: 10.3321/j.issn:1000-7091.1991.03.011.

YangPeiru, WangRuihuan, ZhangJunming, ZhangShu'e. A New Technology of Compound Stain Squash and a New Stain for Identification of Chromosomes[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1991, 6(3): 61-64. doi: 10.3321/j.issn:1000-7091.1991.03.011.

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