牦牛<em>IGF-IR</em>基因克隆及其序列分析

doi:10.7668/hbnxb.2018.04.006

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摘要为研究牦牛胰岛素样生长因子1受体基因（IGF-IR）的结构和功能，采用同源克隆和RT-PCR方法分段扩增牦牛IGF-IR，拼接获得全长序列，利用生物学软件进行分析。结果表明，牦牛IGF-IR基因ORF长约4 104 bp，编码1 367个氨基酸；理论分子量约154.95 ku，等电点pI值约5.71。与黄牛IGF-IR比较，牦牛IGF-IR基因序列有20处碱基改变，其中G到T碱基颠换2次，G到A碱基转换2次，C到T碱基转换7次，A到G碱基转换3次，T到C碱基转换6次，以及3个位点氨基酸改变（G6R、T29I和S30I）。系统进化分析表明，牦牛与黄牛、猪、人、狗、小鼠、猩猩等物种IGF-IR氨基酸相似性均大于92%，处于同一进化分支，具有高度同源性。蛋白结构预测表明，牦牛IGF-IR包含半胱氨酸富集区（FU）、纤连蛋白Ⅲ型结构域（FN3）、跨膜结构域（TM）及酪氨酸蛋白激酶区（TyrKc），具有该受体家族的特征结构域。同源建模表明，其成熟区段氨基酸序列与人的IGF-IR有98.68%的相似性。为后续牦牛IGF-IR基因的功能研究奠定了基础。

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	梁春年
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	阎萍

关键词 ：牦牛； IGF-IR基因；克隆；序列分析

Abstract：The complete cDNA encoding yak insulin like growth factor 1 receptor (IGF-IR) gene was cloned with RT-PCR and homologous cloning methods. Sequence analysis showed that the entire coding region was 4 104 bp in length encoding 1 367 amino acids with a putative mass of 154.95 ku and pI of 5.71. Compared to IGF-IR gene sequence from cattle, there were 20 base changes in yak IGF-IR gene, including 2 substitutions for G-T, 2 transitions for G-A, 7 transitions for C-T, 3 transitions for A-G and 6 transitions for T-C, respectively. In addition, amino acid changed at 3 sites (G6R, T29I and S30I). The phylogenetic analysis revealed that yak IGF-IR gene had high homology (> 92%)with that of cattle, pig, human, dog, mouse and chimpamzee, which were classified into the same branch. The secondary structure predication indicated that yak IGF-IR protein might have the typical domains of the IGF-IR family, containing the furin-like cysteine rich region (FU), fibronectin type Ⅲ domain (FN3), transmembrane domain (TM), and tyrosine kinase domain (TyrKc), respectively. 3D model showed the mature fragment in yak IGF-IR had 98.68% homology with human IGF-IR. The present results provided the valuable foundation for further studies on yak IGF-IR functions.

Key words： Yak ; IGF-IR gene ; Cloning ; Sequence analysis

收稿日期: 2018-02-19

:	Q78
	S823

基金资助:国家重点研发计划项目（2018YFD0502400）；甘肃省草食畜产业技术体系；中国农业科学院牦牛资源与育种创新团队（CAAS-ASTIP-2014-LIHPS）

通讯作者: 阎萍(1963-),女,山西运城人,研究员,博士,主要从事牦牛遗传育种研究。

作者简介: 梁春年(1973-),男,甘肃武威人,研究员,博士,主要从事动物遗传育种研究。

引用本文:

梁春年, 王宏博, 吴晓云, 褚敏, 郭宪, 阎萍. 牦牛IGF-IR基因克隆及其序列分析[J]. 华北农学报, 2018, 33(4): 39-45. doi: 10.7668/hbnxb.2018.04.006.
LIANG Chunnian, WANG Hongbo, WU Xiaoyun, CHU Min, GUO Xian, YAN Ping. Cloning and Sequence Analysis of Yak IGF-IR Gene. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(4): 39-45. doi: 10.7668/hbnxb.2018.04.006.

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http://www.hbnxb.net/CN/10.7668/hbnxb.2018.04.006 或 http://www.hbnxb.net/CN/Y2018/V33/I4/39

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