摘要： 以耐干旱、增产潜力大、抗病毒病、品质优良的晋豆19号大豆为材料,以子叶节、下胚轴、上胚轴、子叶、真叶为外植体,研究外植体类型、激素配比、接种方式等对再生频率的影响。结果显示:无菌苗刚转绿的下胚轴是诱导愈伤组织的最适外植体;诱导愈伤的最适培养基为MS+0.2 mg/L 6-BA+1.0 mg/L 2,4-D;诱导芽分化的最适培养基为MS+1.0 mg/L 6-BA+0.05 mg/L NAA;诱导根分化的最适培养基为MS+0.25 mg/L NAA。各种外植体中,诱导频率高低依次为下胚轴、上胚轴、子叶节、子叶、真叶。

关键词: 大豆, 外植体, 诱导

Abstract: To establish a high frequency regeneration system for the gene transformation of soybean(Glycine?max )，different explants from Jindou No. 19 were studied and the effect of different concentration of hormone and?inoculating ways was also investigated in this study. The results indicated that the hypocotyl from Jindou No. 19 was?more suitable for callus induction of explant in soybeans and the optimal inductive medium was MS+0. 2 mg/L 6BA+1. 0 mg/L 2,4-D. The optimal inductive mediums for shooting and rooting were MS+1.0 mg/L6一BA+0. OS?mg/L NAA and MS+0. 25 mg/L NAA，respectively. In comparison with the different explants regeneration system，?the regeneration frequency was as follows:hypocotyl，epicotyl，cotyledonary node，cotyledon，leaf.

Key words: Soybean, Explants, Induction

中图分类号: 

• S565.1