摘要： 为获得牦牛CTGF基因的CDS区序列及其编码蛋白的结构和功能，并研究该基因在肾脏、心脏、肺脏、肝脏和臀肌等组织中的mRNA表达水平。以类乌齐牦牛为研究对象，采取RT-PCR方法获得类乌齐牦牛CTGF基因CDS序列，荧光定量PCR（qPCR）方法检测该基因在其5个组织中的mRNA表达水平。结果显示：牦牛CTGF基因含2个CpG岛，开放阅读框长度为1 050 bp（登录号：MT968972），可编码349个氨基酸，CTGF编码蛋白存在信号肽，为水溶性不稳定的表面蛋白，共存在21个磷酸化位点和7个糖基化位点，在内质网和微体（过氧物酶体）中分布较多，有3个完整的保守功能结构域。荧光定量结果显示，CTGF基因在类乌齐牦牛肾脏、心脏、肺脏、肝脏和臀肌组织中均有表达，且在肝脏中表达水平最高。旨在为CTGF基因在调控牦牛肌肉生长发育方面提供参考数据。

关键词: 牦牛, CTGF基因, 克隆, 蛋白功能分析, 组织表达分析

Abstract: In order to obtain the CDS region sequence of the yak CTGF gene and the structure and function of the encoded protein, and to study the mRNA expression level of this gene in tissues such as kidney, heart, lung, liver and gluteal muscle. The experiment took Leiwuqi yak as the research object. RT-PCR was used to obtain the CDS sequence of CTGF gene of Leiwuqi yak. Fluorescence quantitative PCR(qPCR)method was used to detect the mRNA expression level of this gene in five tissues. The results showed that the yak CTGF gene contained two CpG islands, the open reading frame length was 1 050 bp(accession number:MT968972), which could encode 349 amino acids, and the CTGF encoded protein had a signal peptide, which was a water-soluble unstable surface protein. There were 21 phosphorylation sites and 7 glycosylation sites, which were more distributed in the endoplasmic reticulum and microsomes(peroxisomes), with 3 complete conserved functional domains. Fluorescence quantitative results showed that CTGF gene was expressed in kidney, heart, lung, liver and gluteal muscle tissues of Leiwuqi yak, and the expression level was highest in liver. The aim was to further understand the role of CTGF gene in regulating yak muscle growth and provide reference data.

Key words: Yak, CTGF gene, Cloning, Protein function analysis, Tissue expression analysis

中图分类号: 

• S823.85