大豆中3个Dof转录因子的生物信息学及表达分析

doi:10.7668/hbnxb.20190032

摘要/Abstract

摘要： 植物Dof转录因子在植物应对非生物胁迫过程中发挥重要作用。为探明Dof转录因子参与大豆对非生物胁迫的响应，对大豆中3个Dof转录因子（GmDof2.1、GmDof3.1和GmDof4.6）的基因序列进行生物信息学分析。3个Dof基因分别位于不同染色体上，它们所编码的蛋白序列长度为212~305个氨基酸残基，均具有1个保守的Dof结构域。3个Dof蛋白主要定位于细胞核中且含有不同数量的磷酸化位点。启动子序列分析表明，GmDof2.1启动子序列中含有3种与逆境和激素响应相关的元件（ARE、DRE1和MBS），GmDof3.1启动子序列中含有6种与逆境和激素响应相关的元件（ABRE、ARE、CGTCA-motif、TGACG-motif、W-box和WUN-motif），GmDof4.6启动子序列中含有7种与逆境和激素响应相关的元件（ABRE、ARE、CGTCA-motif、GARE-motif、MBS、TGACG-motif和WUN-motif）。实时荧光定量PCR结果显示，3个Dof基因均可不同程度的响应高盐、干旱、低温和高温胁迫。GmDof2.1和GmDof3.1在大豆根中的表达量最高，GmDof4.6在大豆茎中的表达量最高。由此推测3个Dof转录因子可能在大豆应对非生物胁迫过程中发挥转录调控作用。

关键词: 大豆, Dof转录因子, 非生物胁迫, 表达分析

Abstract: Dof transcription factors play an important role in plant response to abiotic stress. In order to investigate the Dof transcription factors of soybean involved in the response to abiotic stress, the bioinformatics analysis were performed on three Dof genes(GmDof2.1,GmDof3.1 and GmDof4.6) from soybean, which were located on different chromosomes. The length of the three Dof proteins ranged from 212 to 305 amino acid residues, all of which had a conserved Dof domain. They were mainly located in the nucleus and contained different amounts of phosphorylation sites. Promoter sequence analysis showed that GmDof2.1 promoter contained three cis-acting elements related to stress and hormone(ARE, DRE1 and MBS), GmDof3.1 promoter contained six cis-acting elements related to stress and hormone(ABRE, ARE, CGTCA-motif, TGACA-motif, W-box and WUN-motif), and GmDof4.6 promoter contained seven cis-acting elements related to stress and hormone(ABRE, ARE, CGTCA-motif, GARE-motif, MBS, TGACG-motif and WUN-motif). The results of Real-time fluorescence quantitative PCR showed that all three Dof genes could respond to the stresses of high salt, drought, low temperature and high temperature in varying degrees. GmDof2.1 and GmDof3.1 had the highest expression in roots, and GmDof4.6 had the highest expression in stems. It was inferred that the three Dof transcription factors might play a transcriptional regulatory role in soybean response to abiotic stresses.

Key words: Soybean, Dof transcription factor, Abiotic stress, Expression analysis

中图分类号:

S562.03
Q78

翟莹, 邱爽, 张军, 刘春雨, 赵艳, 李珊珊, 张梅娟, 邱增城, 任巍巍. 大豆中3个Dof转录因子的生物信息学及表达分析[J]. 华北农学报, 2019, 34(6): 14-19. doi: 10.7668/hbnxb.20190032.

ZHAI Ying, QIU Shuang, ZHANG Jun, LIU Chunyu, ZHAO Yan, LI Shanshan, ZHANG Meijuan, QIU Zengcheng, REN Weiwei. Bioinformatics and Expression Analysis of Three Dof Transcription Factors in Soybean[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(6): 14-19. doi: 10.7668/hbnxb.20190032.

http://www.hbnxb.net/CN/Y2019/V34/I6/14

参考文献

[1] Yanagisawa S.Dof domain proteins:plant-specific transcription factors associated with diverse phenomena unique to plants[J].Plant&Cell Physiology,2004,45(4):386-391. doi:10.1093/pcp/pch055.
[2] Yanagisawa S, Izui K. Molecular cloning of two DNA-binding proteins of maize that are structurally different but interact with the same sequence motif[J]. The Journal of Biological Chemistry, 1993, 268(21):16028-16036.
[3] Kumar R, Taware R, Gaur V S, Guru S K, Kumar A.Influence of nitrogen on the expression of TaDof1 transcription factor in wheat and its relationship with photo synthetic and ammonium assimilating efficiency[J].Molecular Biology Reports,2009,36(8):2209-2220.doi:10.1007/s11033-008-9436-8.
[4] Li D J, Yang C H, Li X B, Gan Q, Zhao X F, Zhu L H.Functional characterization of rice OsDof12[J].Planta,2009,229(6):1159-1169.doi:10.1007/s00425-009-0893-7.
[5] Venkatesh J, Park S W.Genome-wide analysis and expression profiling of DNA-binding with one zinc finger (Dof) transcription factor family in potato[J].Plant Physiology and Biochemistry,2015,94:73-85.doi:10.1016/j.plaphy.2015.05.010.
[6] 杨桂燕,赵震,赵玉琳,张凤娇,高彩球. ThMYB3和ThDof2对ThVHAc1基因表达的调控[J].北京林业大学学报, 2015, 37(12):1-6. doi:10.13332/j.1000-1522.20140486.
Yang G Y, Zhao Z, Zhao Y L, Zhang F J, Gao C Q. Upstream regulators of ThVHAc1[J]. Journal of Beijing Forestry University, 2015,37(12):1-6.
[7] 卫晓彬,王亚楠,张超,单丽伟,唐如春,范三红.小麦WPBF与高分子量谷蛋白基因上游Prolamin-Like box的特异结合[J].中国农业科学, 2012, 45(1):7-15. doi:10.3864/j.issn.0578-1752.2012.01.002.
Wei X B, Wang Y N, Zhang C, Shan L W, Tang R C, Fan S H. Specific binding of wheat WPBF and Prolamin-Like box in upstream of HMW-GS genes[J]. Scientia Agricultura Sinica, 2012,45(1):7-15.
[8] Gualberti G, Papi M, Bellucci L, Ricci L, Bouchez D, Camilleri C, Costantino P, Vittorioso P.Mutations in the Dof zinc finger genes DAG2 and DAG1 influence with opposite effects the germination of Arabidopsis seeds[J].The Plant Cell,2002,14(6):1253-1263.doi:10.1105/tpc.010491.
[9] Yanagisawa S, Akiyama A, Kisaka H, Uchimiya H, Miwa T.Metabolic engineering with Dof1 transcription factor in plants:improved nitrogen assimilation and growth under low-nitrogen conditions[J].Proceedings of the National Academy of Sciences of the United States of America,2004,101(20):7833-7838.doi:10.1073/pnas.0402267101.
[10] Huang W, Huang Y, Li M Y, Wang F, Xu Z S, Xiong A S.Dof transcription factors in carrot:genome-wide analysis and their response to abiotic stress[J].Biotechnology Letters,2016,38(1):145-155.doi:10.1007/s10529-015-1966-2.
[11] Gupta S, Malviya N, Kushwaha H, Nasim J, Bisht N C, Singh V K, Yadav D.Insights into structural and functional diversity of Dof (DNA binding with one finger) transcription factor[J].Planta,2015,241(3):549-562.doi:10.1007/s00425-014-2239-3.
[12] Shaw L M, Mcintyre C L, Gresshoff P M, Xue G P.Members of the Dof transcription factor family in Triticum aestivum are associated with light-mediated gene regulation[J].Functional∬egrative Genomics,2009,9(4):485-498.doi:10.1007/s10142-009-0130-2.
[13] Ma J, Li M Y, Wang F, Tang J, Xiong A S.Genome-wide analysis of Dof family transcription factors and their responses to abiotic stresses in Chinese cabbage[J].BMC Genomics,2015,16:33.doi:10.1186/s12864-015-1242-9.
[14] Corrales A R, Nebauer S G, Carrillo L, Fernández-Nohales P, Marqués J, Renau-Morata B, Granell A, Pollmann S, Vicente-carbajosa J, Molina R V, Medina J.Characterization of tomato cycling Dof factors reveals conserved and new functions in the control of flowering time and abiotic stress responses[J].Journal of Experimental Botany,2014,65(4):995-1012.doi:10.1093/jxb/ert451.
[15] 吴智明,张圣旭,梁关生.马铃薯基因组中Dof转录因子家族的鉴定与表达特征分析[J].核农学报, 2015, 29(7):1260-1270. doi:10.11869/j.issn.100-8551.2015.07.1260.
Wu Z M, Zhang S X, Liang G S. Genome-wide identification and expression analysis of the Dof transcription factor family in potato (Solanum tuberosum L.)[J]. Journal of Nuclear Agricultural Sciences, 2015,29(7):1260-1270.
[16] 李辉,黄蔚,刘志薇,王永鑫,吴致君,庄静.茶树两个Dof转录因子的分离及其在温度胁迫中的响应分析[J].茶叶科学, 2016, 36(3):312-322.doi:10.13305/j.cnki.jts.2016.03.011.
Li H, Huang W, Liu Z W, Wang Y X, Wu Z J, Zhuang J. Isolation and expression analysis of two temperature responsive Dof genes from Camellia sinensis[J]. Journal of Tea Science, 2016,36(3):312-322.
[17] Zhang J H, Hao Q, Bai L L, Xu J, Yin W B, Song L Y, Xu L, Guo X J, Fan C M, Chen Y H,Ruan J,Hao S T,Li Y G,Wang R R C,Hu Z M.Overexpression of the soybean transcription factor GmDof4 significantly enhances the lipid content of Chlorella ellipsoidea[J].Biotechnology for Biofuels,2014,7(1):10. doi:10.1186/s13068-014-0128-4.
[18] Wang H W, Zhang B, Hao Y J, Huang J, Tian A G, Liao Y, Zhang J S, Chen S Y.The soybean Dof-type transcription factor genes, GmDof4 and GmDof11, enhance lipid content in the seeds of transgenic Arabidopsis plants[J].The Plant Journal:for Cell and Molecular Biology,2007,52(4):716-729.doi:10.1111/j.1365-313X.2007.03268.x.
[19] Zhao T J, Sun S, Liu Y, Liu J M, Liu Q, Yan Y B, Zhou H M. Regulating the drought-responsive element (DRE)-mediated signaling pathway by synergic functions of trans-active and trans-inactive DRE binding factors in Brassica napus[J].Journal of Biological Chemistry,2006,281(16):10752-10759. doi:10.1074/jbc.M510535200.
[20] Zhuang J, Jiang H H, Wang F, Peng R H, Yao Q H, Xiong A S.A rice OsAP23, functioning as an AP2/ERF transcription factor, reduces salt tolerance in transgenic Arabidopsis[J].Plant Molecular Biology Reporter,2013,31(6):1336-1345.doi:10.1007/s11105-013-0610-3.
[21] Skirycz A, Jozefczuk S, Stobiecki M, Muth D,Zanor M I, Witt I, Roeber B M.Transcription factor AtDOF4;2 affects phenylpropanoid metabolism in Arabidopsis thaliana[J].New Phytologist,2007,175(3):425-438. doi:10.1111/j.1469-8137.2007.02129.x.
[22] Kang H G, Singh K B.Characterization of salicylic acid-responsive, Arabidopsis Dof domain proteins:overexpression of OBP3 leads to growth defects[J].The Plant Journal,2000,21(4):329-339.doi:10.1046/j.1365-313x.2000.00678.x.
[23] 蔡晓锋,张余洋,张俊红,李汉霞,叶志彪.植物Dof基因家族功能研究进展[J].植物生理学报, 2013, 49(1):1-12.doi:10.13592/j.cnki.ppj.2013.01.012.
Cai X F, Zhang Y Y, Zhang J H, Li H X, Ye Z B. Advances in research on function of the Dof gene family in plant[J]. Acta Phytophysiologica Sinica, 2013,49(1):1-12.
[24] 刘文婷,马田田,周春菊,藏潇,李浪金,张宝军,杜威,张炜丽,陈坤明.禾本科植物单锌指家族基因对逆境应答的研究进展[J].生物工程学报, 2016, 32(5):541-553. doi:10.13345/j.cjb.150351.
Liu W T, Ma T T, Zhou C J, Zang X, Li L J, Zhang B J, Du W, Zhang H L, Chen K M. Advances in stress response of DNA binding with one finger transcription factor family genes in graminaceous plants[J]. Chinese Journal of Biotechnology, 2016,32(5):541-553.
[25] Shinozaki K, Yamaguchi-Shinozaki K, Seki M.Regulatory network of gene expression in the drought and cold stress responses[J].Current Opinion in Plant Biology,2003,6(5):410-417.doi:10.1016/S1369-5266(03)00092-X.
[26] 周淑芬,颜静宛,刘华清,林智敏,陈睿,杨绍华,王锋.水稻Dof基因家族的组织表达谱及胁迫诱导表达特征分析[J].分子植物育种, 2012, 10(6):635-643. doi:10.3969/mpb.010.000635.
Zhou S F, Yan J W, Liu H Q, Lin Z M, Chen R, Yang S H, Wang F. Transcriptional profiling analysis of OsDof gene family in various rice tissues and their expression characteristics under different stresses[J]. Molecular Plant Breeding, 2012,10(6):635-643.

选择文件类型/文献管理软件名称

选择包含的内容