摘要： 为探明MAP30基因的表达调控规律，从苦瓜种质Y5中克隆了MAP30基因上游包括起始密码子在内1 682 bp序列，选取转录起始位点上游1 500 bp序列利用PlantCARE在线启动子预测工具进行分析，结果表明：MAP30启动子除了含有核心元件TATA-box和CAAT-box外，还含有大量的光响应元件、植物激素脱落酸响应元件、热胁迫响应元件、干旱应答元件、水杨酸应答元件等，该预测结果表明，MAP30启动子可能参与光、厌氧、逆境胁迫、脱落酸、水杨酸和乙烯等诱导，同时它还可能参与胚乳高效表达及昼夜节律调控。以30份苦瓜种质为材料，分析了MAP30启动子区域SNPs和InDels分布情况，共发现37个SNPs位点和6个InDels，SNPs分组发现，30份苦瓜种质资源MAP30基因启动子共存在3种单倍型，Y5、Y7、Y16、Y39、Y58、Y60、Y66、Y77、Y83、Y96、Y100、Y112、Y121属于第1组，Y43、Y50、Y90、Y108、Y113、Y115、Y140、Y141、Y144属于第2组，Y69、Y72、Y85、Y131、Y139、Y146、Y147、Y153属于第3组。结合顺式调控元件的位置，其中5个SNPs，342位点的T/C，584位点的A/G，783位点的G/T，872位点的A/G，1087位点的T/G，位于顺式调控元件内部，涉及的顺式调控元件分别为MBSI、ABRE、ARE、5UTR Py-rich stretch、GAG-motif、TC-rich repeats，SNP导致转录因子结合位点发生改变，可能对MAP30基因的表达调控有重要作用。为进一步揭示MAP30基因转录调控机制奠定理论基础。

关键词: 苦瓜, MAP30, 启动子, 单倍型

Abstract: In order to explore the expression regulation of MAP30 gene,the upstream sequence,1 682 bp in length including start codon of MAP30 gene was cloned in bitter gourd (Y5).1 500 bp upstream of the transcription start site was selected for analysis based on PlantCARE promoter prediction tools.The results showed that in addition to containing the core elements of TATA-box and CAAT-box,the MAP30 promoter also contained light response elements,abscisic acid response element,heat stress response element,drought response element,salicylic acid response element etc.The prediction results suggested that MAP30 promoter might be involved in the induction of light, anaerobic, stress, abscisic acid, salicylic acid and ethylene, and it might also play important roles in the efficient expression in endosperm and circadian rhythm regulation. Take 30 bitter gourds germplasm as materials,the SNPs and InDels distribution in promoter region of MAP30 was analyzed.37 SNPs loci and 6 InDels were found.3 haplotypes were identified in MAP30 gene promoter through SNPs grouping.Y5, Y7, Y16, Y39, Y58, Y60, Y66, Y77, Y83, Y96, Y100, Y112 and Y121 belonged to group 1, Y43, Y50, Y90, Y108, Y113, Y115, Y140, Y141 and Y144 belonged to group 2, Y69, Y72, Y85, Y131, Y139, Y146, Y147 and Y153 belonged to group 3. Combining with the positions of cis regulatory elements, 5 SNPs, including T/C in 342 loci, A/G in 584 loci, G/T in 783 loci, A/G in 872 loci, T/G in 1087 loci, located in the cis regulatory elements region, the involving cis regulatory elements were MBSI, ABRE, ARE, 5UTR Py-rich stretch, GAG-motif and TC-rich repeats. The changes of the transcription factor binding sites caused by SNP may play important roles in regulating the expression of MAP30 gene. This study laid a theoretical foundation for further revealing the transcription regulation mechanism of MAP30 gene.

Key words: Bitter gourd, Momordica anti-HIV protein of 30 kDa, Promoter, Haplotype

中图分类号: 

• Q78

• S642.5