NaCl胁迫下外源壳聚糖对菜用大豆光合作用及叶绿体活性氧代谢的影响

doi:10.7668/hbnxb.2016.04.026

摘要/Abstract

摘要： 采用蛭石栽培，以菜用大豆为试材，研究外源壳聚糖对NaCl胁迫下幼苗叶片光合作用及叶绿体活性氧（ROS）代谢的影响，以期探明NaCl胁迫下壳聚糖调控菜用大豆光合作用的生理机制。结果显示：NaCl胁迫下，外源壳聚糖通过诱导非气孔因素显著缓解了菜用大豆在胁迫中期（第6～12天）净光合速率（Pn）的下降；外源壳聚糖显著抑制了叶绿体O₂O₂^·的产生速率及H₂O₂含量的上升，显著提高了超氧化物歧化酶（SOD）、过氧化物酶（POD）、谷胱甘肽还原酶（GR）活性及抗坏血酸过氧化物酶（APX）在胁迫中期（第6～12天）的活性。无盐条件下，外源壳聚糖在处理早期对上述各指标产生了显著影响，中、后期该作用消失。上述结果表明：外源壳聚糖在NaCl胁迫下对菜用大豆的作用与无盐条件下不同；NaCl胁迫下，壳聚糖可诱导菜用大豆叶绿体保护酶活性及AsA-GSH循环的运转速率，及时清除过量ROS，以抑制盐胁迫对光合膜的伤害，这可能是壳聚糖缓解Pn下降，进而缓解菜用大豆干质量下降的重要原因之一；外源壳聚糖的作用具有时效性。

关键词: 外源壳聚糖, NaCl胁迫, 光合作用, 叶绿体活性氧代谢

Abstract: In order to investigate the mechanism of chitosan on regulating photosynthesis under NaCl stress,the effects of exogenous chitosan on photosynthesis and chloroplast active oxygen metabolism in seedling leaves of vegetable soybean under NaCl stress were studied by adopting vermiculite culture.The results showed that exogenous chitosan alleviated the decline of net photosynthetic rate (Pn) markedly at the 6th,9th and 12th days of salinity by inducing non stomatal factors under NaCl stress.Exogenous chitosan significantly decreased chloroplast O₂O₂^· production rate and H₂O₂ contents,and increased the activities of superoxide dismutase (SOD),peroxidase (POD),glutathione reductase (GR) markedly,meanwhile the ascorbate peroxidase (APX) activity during the mid-term (the 6th to 12th day) of NaCl stress also was increased.Under salt free conditions,exogenous chitosan had significant effects on the above indexes in the early stage of treatment,but the effect disappeared in the mid and late period.The results indicated that the effects of exogenous chitosan under NaCl stress were different to those of salt free conditions;Chitosan induced the protective enzyme activity and the running rate of AsA-GSH cycle of soybean chloroplast and then removed excess reactive oxygen species in time so that inhibit the damage of the photosynthetic membrane.This might be the main reason for chitosan alleviate Pn decreasing,and then alleviate the decline in the dry weight of vegetable soybean;The effect of exogenous chitosan was time-sensitive.

Key words: Exogenous chitosan, NaCl stress, Photosynthesis, Chloroplast reactive oxygen species metabolism

中图分类号:

S643.01

王聪, 杨恒山, 董永义, 马玉露, 贾俊英, 包金花, 郑毅. NaCl胁迫下外源壳聚糖对菜用大豆光合作用及叶绿体活性氧代谢的影响[J]. 华北农学报, 2016, 31(4): 162-167. doi: 10.7668/hbnxb.2016.04.026.

WANG Cong, YANG Hengshan, DONG Yongyi, MA Yulu, JIA Junying, BAO Jinhua, ZHENG Yi. Effects of Exogenous Chitosan on Photosynthesis and Chloroplast Reactive Oxygen Species Metabolism of Vegetable Soybean under NaCl Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(4): 162-167. doi: 10.7668/hbnxb.2016.04.026.

http://www.hbnxb.net/CN/Y2016/V31/I4/162

参考文献

[1] Guan Y J,Hu J,Wang X J,et al.Seed priming with chitosan improves maize germination and seedling growth in relation to physiological changes under low temperature stress[J].Journal of Zhejiang University-Science B,2009,10(6):427-433.
[2] Zhao T H,Wang J L,Wang Y,et al.Effects of reactive Oxygen species metabolic system on soybean (Glycine max) under exogenous chitosan to ozone stress[J].Bulletin of Environmental Contamination and Toxicology,2010,85(1):59-63.
[3] 马彦霞,郁继华,张国斌,等.壳聚糖对水分胁迫下辣椒幼苗氧化损伤的保护作用[J].中国农业科学,2012,45(10):1964-1971.
[4] Pongprayoon W,Roytrakul S,Pichayangkura R,et al.The role of Hydrogen peroxide in chitosan-induced resistance to osmotic stress in rice (Oryza sativa L.)[J].Plant Growth Regulation,2013,70(2):159-173.
[5] 周娟,邹礼平.壳聚糖对盐胁迫下豇豆幼苗生长发育的影响[J].北方园艺,2014(23):30-33.
[6] 杨华庚,杨毅敏,廖红妮,等.壳聚糖对蝴蝶兰幼苗耐热性的诱导作用[J].生态学杂志,2015,34(12):3430-3437.
[7] 蒋先明.蔬菜栽培学总论[M].北京:中国农业出版社,2004:191.
[8] 王聪,朱月林,杨立飞.菜用大豆耐盐品种的筛选及其耐盐生理特性[J].江苏农业学报,2009,25(3):621-627.
[9] 孙锦.菠菜对海水胁迫响应的生理机制研究[D].南京:南京农业大学,2009:61-62.
[10] Takeda K,Otaubo T,Konda N.Participation of hydrogen peroxide in the inactivation of Calvin-cycle GSH enzyme in SO₂-furmugated spinach leaves[J].Plant Cell Physiol,1982,23:1009-1018.
[11] Farquhar G D,Sharkey T D.Stomatal conductance and photosynthesis[J].Ann Rev Plant Physiol,1982,33:317-345.
[12] Khan W M,Prithiviraj B,Smith D L.Effect of foliar application of chitin and chitosan oligosaccharides on photosynthesis of maize and soybean[J].Photosynthetica,2002,40(4):621-624.
[13] 寇伟锋,刘兆普,陈铭达,等.不同浓度海水对油葵幼苗光合作用及叶绿素荧光特性的影响[J].西北植物学报,2006,26(1):73-77.
[14] 尹赜鹏,刘雪梅,商志伟,等.不同干旱胁迫下欧李光合及叶绿素荧光参数的响应[J].植物生理学报,2011,47(5):452-458.
[15] Wong S C,Cowan I R,Farquhar G D.Leaf conductance in relation to rate of CO₂ assimilation:Ⅲ.influences of water stress and photoinhibition[J].Plant Physiology,1985,78(4):830-834.
[16] 刘建新,胡浩斌,王鑫.硅对盐胁迫下黑麦草幼苗活性氧代谢和光合参数的影响[J].中国草地学报,2008,30(5):25-31.
[17] 车永梅,唐静,陈康,等.一氧化氮对盐胁迫下玉米幼苗叶绿素荧光特性及光合作用的影响[J].玉米科学,2009,17(3):91-94.
[18] Cheeseman J M.Mechanisms of salinity tolerance in plants[J].Plant Physiology,1988,87(3):547-550.
[19] Cakmak I.Activity of ascorbate-pendent H₂O₂ scavenging enzymes and leaf chlorosis are enhanced in magnesium and potassium deficient leaves,but not in phosphorus deficient leaves[J].J Exp Bot,1994,45:1259-1266.
[20] Povero T,Loreti E,Pucciariello C,et al.Transcript profiling of chitosan-treated Arabidopsis seedlings[J].J Plant Res,2011,124:619-629.
[21] Liu T,Liu Z,Song C,et al.Chitin-induced dimerization activates a plant immune receptor[J].Science,2012,336(685):1160-1164.
[22] Chinnusamy V,Jagendorf A,Zhu J K.Understanding and improving salt tolerance in plants[J].Crop Science,2005,45(2):437-448.
[23] Link V L,Hofmann M G,Sinha A K,et al.Biochemical evidence for the activation of distinct subsets of mitogen-activated protein kinases by voltage and defense-related stimuli[J].Plant Physiology,2002,128(1):271-281.

选择文件类型/文献管理软件名称

选择包含的内容