摘要： 为了解旱黄瓜种质资源的遗传多样性及亲缘关系,利用黄瓜全基因组测序的450对SSR引物对38份旱黄瓜种质资源进行了多样性和聚类分析.结果表明:72对引物共扩增出133个等位变异,平均每个SSR位点的等位基因数为1.85个,变幅为1~4个.38份旱黄瓜种质资源的相异系数分布在0.215~0.704,其中6457与佳美旱黄瓜、选丰与佳美旱黄瓜、新宝与绿之秋亲缘关系较远,而gfcs与四季翠绿、玉翡翠与翠玉、真把握与唐山秋瓜、真把握与皇家绿秀亲缘关系较近.在阈值为0.54处,可将这38份黄瓜种质资源划分为Ⅰ、Ⅱ类.在阈值为0.48处,可将Ⅰ类再分为Ⅰ_A、Ⅰ_B、Ⅰ_C、Ⅰ_D 4个亚类,Ⅱ类可再分为Ⅱ_A、Ⅱ_B、Ⅱ_C 3个亚类.为旱黄瓜育种中亲本的选择选配提供了参考.

关键词: 黄瓜, 种质资源, 亲缘关系, 遗传多样性, SSR

Abstract: Drought cucumber is one of the characteristics of industrial advantages of Jidong area.To evaluate the genetic diversity and relationship of cucumber germplasm,38 South-China ecotype cucumber accessions were subjected to analysis of diversity and cluster via 450 paires of SSR markers.72 SSR primers generated 133 polymorphic bands on an average of 1.85 polymorphic bands per primer pairs(ranged from 1 to 4).The index of dissimilarity of 38 cucumber germplasm accessions varied from 0.215 to 0.704.A close genetic relationship was found between Sijicuilü and gfcs,Yufeicui and Cuiyu,Tangshanqiugua and Zhenbawo,and between Zhenbawo and Huangjialüxiu.But the genetic relationship between Jiameihanhuanggua and Xuanfeng or 6457,and between Xinbao and Lüzhiqiu were relatively distant.The clustering analysis showed that the 38 accessions were divided into two groups,group Ⅰ and Ⅱ,at a threshold 0.54.Further,the group Ⅰ was subdivided intoⅠ_A,Ⅰ_B,Ⅰ_C and Ⅰ_D subgroups while the group Ⅱ was subdivided intoⅡ_A,Ⅱ_B and Ⅱ_C at the threshold 0.48.The results were helpful to the parental selection and cross planning in breeding programs for South-China cucumber.

Key words: Cucumis sativus L., Genetic resources, Genetic relationship, Genetic diversity, SSR

中图分类号: 

• S642.03