摘要： 为了给筛选萼脊兰内参基因和研究TUB基因的生理功能提供研究基础,对萼脊兰TUB基因片段进行克隆与序列分析。采用CTAB法提取萼脊兰盛花期花瓣总RNA,并运用RT-PCR方法克隆萼脊兰TUB基因序列,长度为1 055 bp,编码351个氨基酸残基,氨基酸序列中存在微管蛋白的保守区域GGGTGSG。序列分析结果表明:与其他已登录物种的TUB基因相比,其核苷酸序列的同源性达75% ～96%,与朵丽蝶兰(JN185665.1)同源性高达96%,氨基酸序列的同源性也在96%以上,且与单子叶植物的同源序列表现出较高的相似性。在GenBank注册,登录号为KP686397。

关键词: 萼脊兰, TUB 基因, 克隆, 序列分析

Abstract: TUB of Sedirea japonica was cloned and sequence analyzed,and the result would provide an important basis for selecting a reference gene and studying the function of TUB.The total RNA of Sedirea japonica scapes was extracted by CTAB method and the partial sequence of Sedirea japonica TUB was cloned by RT-PCR,which was 1 055 bp long and encoded 351 amino acids residues.Its amino acid sequence contained a conservative region GGGTGSG.The sequencing result revealed that the TUB gene fragment from Sedirea japonica homology comparison with other plants TUB gene sequences in the GenBank shows that they share 75%-96% nucleotide sequence homology,96% nucleotide sequence homology with Doritaenopsis hybrid TUB,the amino acid sequence homology is above 96% and amino acid sequence homology high similarity with the monocotyledon.The cloned genes were TUB gene fragment,which were registered them into GenBank(respective accession number:KP686397).

Key words: Sedirea japonica, TUB gene, Cloning, Sequence analysis

中图分类号: 

• Q786