冷诱导下毛百合甘油-3-磷酸酰基转移酶活性变化及其基因保守区的克隆

doi:10.7668/hbnxb.2011.05.008

华北农学报 ›› 2011, Vol. 26 ›› Issue (5): 34-39. doi: 10.7668/hbnxb.2011.05.008

所属专题： 生物技术；

冷诱导下毛百合甘油-3-磷酸酰基转移酶活性变化及其基因保守区的克隆

陈丽静¹, 葛菱¹, 李浩戈¹, 郭志富¹, 张丽¹, 党晓璇¹, 陶承光², 李天来¹

1. 沈阳农业大学辽宁省生物技术重点实验室设施园艺省部共建教育部重点实验室, 辽宁沈阳110866;
2. 辽宁省农业科学院, 辽宁沈阳110866

收稿日期:2011-05-01 出版日期:2011-10-28
通讯作者: 李天来( 1955-) ，男，辽宁绥中人，教授，博士，博士生导师，主要从事蔬菜设施栽培研究。
作者简介:陈丽静( 1971-) ，女，山东海阳人，副教授，博士，主要从事植物基因工程与细胞工程研究。
基金资助:
辽宁省教育厅项目(L2010495);中国博士后科学基金面上项目资助(20100471471);国家高技术研究发展计划(“863”计划)项目(2006AA100109)

Change in Glycerol-3-Phosphate Acyltransferase Activity of Lilium dauricum Ker Grawl.and Cloning of Its Conserved cDNA Region under Cold Stress

CHEN Li-jing¹, GE Ling¹, LI Hao-ge¹, GUO Zhi-fu¹, ZHANG Li¹, DANG Xiao-xuan¹, TAO Cheng-guang², LI Tian-lai¹

1. Bioscience and Technology Institute and Key Laboratory of Protected Horticulture(Ministry of Education), Shenyang Agricultural University, Shenyang 110866, China;
2. The Liaoning Academy of Agricultural Sciences, Shenyang 110161, China

Received:2011-05-01 Published:2011-10-28

摘要/Abstract

摘要： 探讨了野生毛百合甘油-3-磷酸酰基转移酶活性在冷胁迫下的变化,用简并引物扩增了甘油-3-磷酸酰基转移酶基因保守区序列的结果表明,该保守区段长744 bp,推测编码247个氨基酸。在GPAT氨基酸序列中存在1个高度保守的区域(WIAPSGGRDRP),在NCBI上经过BLASTp比对分析,发现这段保守区序列为LPLAT基因超级家族酶类的催化活性区,此家族多为催化酰基辅酶A(acylCoAs)或者酰基载体蛋白(acylACPs)中的酰基与受体蛋白结合的酰基转移酶类。此序列Genbank登录号为HQ654523。

关键词: 毛百合, 冷诱导, 甘油-3-磷酸酰基转移酶(GPAT), 简并引物, 保守区, 同源性

Abstract: Change in Glycerol-3-Phosphate Acyltransferase(GPAT)activity of Lilium dauricum under cold stress was explored and the conserved sequence of GPAT was amplified using degenerate primers.The results demonstrated that the conserved sequence was 744 bp in length,which was deduced coding 247 amino acids.There is a highly conserved domain(WIAPSGGRDRP)in the amino acid sequence of GPAT.Aligning the conserved domain with BLAST of NCBI,we found it was a catalytic domain of LPLAT gene superfamily enzymes.This family mostly catalyzed acyl-coenzyme A(acylCoAs)or acyltransferase which was acyl in the Acyl carrier protein(acylACPs)binding with receptor protein.The gene accession nucleotide sequence number in GenBank was bankit1413285.

Key words: Lilium dauricum, Cold stress, Glycerol-3-Phosphate acyltransferase, Degenerate primers, Conserved cDNA region, Homology

中图分类号:

S682.29

陈丽静, 葛菱, 李浩戈, 郭志富, 张丽, 党晓璇, 陶承光, 李天来. 冷诱导下毛百合甘油-3-磷酸酰基转移酶活性变化及其基因保守区的克隆[J]. 华北农学报, 2011, 26(5): 34-39. doi: 10.7668/hbnxb.2011.05.008.

CHEN Li-jing, GE Ling, LI Hao-ge, GUO Zhi-fu, ZHANG Li, DANG Xiao-xuan, TAO Cheng-guang, LI Tian-lai. Change in Glycerol-3-Phosphate Acyltransferase Activity of Lilium dauricum Ker Grawl.and Cloning of Its Conserved cDNA Region under Cold Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(5): 34-39. doi: 10.7668/hbnxb.2011.05.008.

http://www.hbnxb.net/CN/Y2011/V26/I5/34

参考文献

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[8] Ariizumi T,Kishitani S,Inatsugi R,et al.An increase in unsaturation of fatty acids in phosphatidylglycerol from leaves improves the rates of photosynthesis and growth at low temperatures in transgenic rice seedlings[J].Plant Cell Physiol,2002,43 (7):751-758.
[9] Frentzen M,Heins E,Mekeon A,et al.Specificatives and selectivities of glycerol-3-phosphate acyltransferase from pea and spinach chloroplasts[J].FEBS Lett,1983,129:629-636.
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冷诱导下毛百合甘油-3-磷酸酰基转移酶活性变化及其基因保守区的克隆

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