摘要： 选用22个稻瘟菌鉴别菌株,采用离体叶片接种法对吉林省全部水稻主栽品种及育种材料(98个)的抗瘟性进行了鉴定.结果显示,吉林省主栽水稻品种对稻瘟病的抗性较弱,高达98%的供试品种均可以被稻瘟菌不同程度地侵染;本研究筛选到3个品种:超级稻2号、吉粳801、吉粳806,具有极高的抗瘟性,为将来克隆新的抗瘟基因及培育新的抗瘟品种提供了宝贵的材料.根据上述抗性鉴定结果,本研究进一步通过PCR方法,以水稻DNA为模板,扩增6个水稻抗瘟基因:Pib、Pi-ta、Pi9、Pi-d2、Pi36及Pikh,检测抗瘟基因在98个品种中的分布情况.结果显示,这6个抗瘟基因的分布频率分别为2.%,94.9%,21.4%,92.9%,86.7%,98%,其中,Pi9和Pib两基因在供试品种中发生了显著缺失,吉林省近年来稻瘟病发病比较严重可能与该缺失存在密切关系.本研究为推广以抗瘟基因多样性为理论的品种布局技术提供了有力的数据支持.
关键词:
水稻品种,
稻瘟病,
抗瘟基因,
稻瘟菌
Abstract: In recent years,rice blast epidemics have seriously occurred in Jilin province of China.Here,the resistance of all Jilin rice varieties to rice blast were analyzed by inoculation with 22 Magnaporthe oryzae strains.The results showed that the blast resistances were weak,because up to 98% of varieties could be colonized by no less than one test strain.Three varieties:Chaojidao 2,Jijing 801 and Jijing 806,were identified with quite high resistance to test strains,and could be used as stocks for cloning of new resistance genes in future.Additionally,six rice blast resistance genes:Pib,Pi-ta,Pi9,Pi-d2,Pi36 and Pikh were analyzed in all the above rice varieties with PCR amplification,and the results showed that Pi9 and Pib existed rarely,which to some extent explains why rice blast disease has occurred seriously in Jilin in past several years.The study facilitates controlling of rice blast with diversity of resistance genes.
Key words:
Rice variety,
Rice blast,
Resistance gene,
Magnaporthe oryzae
中图分类号:
靳春鹏, 孙庚, 刘金亮, 李桂华, 张世宏, 潘洪玉. 吉林省水稻品种对稻瘟病的抗性分析[J]. 华北农学报, 2011, 26(3): 214-218. doi: 10.7668/hbnxb.2011.03.042.
JIN Chun-peng, SUN Geng, LIU Jin-liang, LI Gui-hua, ZHANG Shi-hong, PAN Hong-yu. Identification of Rice Varieties Resistant to Rice Blast in Jilin Province[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(3): 214-218. doi: 10.7668/hbnxb.2011.03.042.