摘要: 以β-肌动蛋白(β-actin)mRNA的表达量作为内标,应用半定量RT-PCR法,对高密度养殖条件下不同月龄革胡子鲶垂体生长激素(Growthhormone,GH)mRNA的相对表达量进行分析。结果显示:3.5,5,6.5月龄试验鱼的GHmRNA的相对表达量分别为3.612~5.965,2.929~4.556和1.360~1.821;平均相对表达量分别为4.346±0.6560,3.649±0.5684和1.575±0.1355。上述数据经方差分析得出,革胡子鲶任意两个不同月龄之间GHmRNA相对表达量的差异达到1%的显著水平。Duncan氏多重比较结果显示,3.5,5月龄试验鱼垂体GHmRNA的平均相对表达量差异不显著,但均极显著高于6.5月龄。
关键词:
革胡子鲶,
生长激素(GH),
半定量RT-PCR
Abstract: Using 3ctiu mRNA expression quantity as an internal reference,the differences of growth hormone(GH) mRNA expression in pituitary of Clarias gariepiuus in high density pond culture at different months old were an-alyzed with the employment of semiuantitative RTCR method.
Key words:
Clarias ariepiuus,
Growth hormone,
Semiuantitative RTCR
中图分类号:
王晓梅, 戴伟, 郭永军, 陈承勋, 李娜, 张茂群. 高密度养殖下革胡子鲶生长激素mRNA表达的半定量分析[J]. 华北农学报, 2011, 26(1): 94-98. doi: 10.7668/hbnxb.2011.01.020.
WANG Xiao-mei, DAI Wei, GUO Yong-jun, CHEN Cheng-xun, LI Na, ZHANG Mao-qun. Semi}uantitative Analysis of Growth Hormone(GH) mRNAExpression of Clarias gariepinus in High Density Pond Culture[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(1): 94-98. doi: 10.7668/hbnxb.2011.01.020.