华北农学报 ›› 2006, Vol. 21 ›› Issue (5): 39-41. doi: 10.3321/j.issn:1000-7091.2006.05.010

所属专题: 小麦 生物技术

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小麦糖原合成酶激酶基因(TaGSK1)的染色体定位

李亚青1,2, 毛新国3, 赵宝存1, 葛荣朝1, 沈银柱1, 黄占景1   

  1. 1. 河北师范大学生命科学学院, 河北石家庄 050016;
    2. 石家庄市农科院, 河北石家庄 050041;
    3. 中国农业科学院作物科学研究所农作物基因资源与基因改良国家重大科学工程农业部作物种质资源与生物技术重点开放实验室, 北京 100081
  • 收稿日期:2006-05-16 出版日期:2006-10-28
  • 通讯作者: 黄占景
  • 作者简介:李亚青(1973- ),女,河北石家庄人,硕士,主要从事植物分子生物学研究工作。
  • 基金资助:
    国家自然科学基金(30070471);国家转基因专项(JY04-A-01);河北省自然科学基金(301103)

The Chromosomal Location of Triticum aestivum Glycogen Synthase Kinase Gene (TaGSK1)

LI Ya-qing1,2, MAO Xin-guo3, ZHAO Bao-cun1, GE Rong-chao1, SHEN Yin-zhu1, HUANG Zhan-jing1   

  1. 1. College of Life Science, Hebei Normal University, Shijiazhuang 050016, China;
    2. Shijiazhuang Institute of Agricultral Sciences, Shijiazhuang 050041, China;
    3. National Key Facility for crop Gene Resources and Genetic Improvement, Key Laboratory of Crop Germplasm and Biotechnology, the Ministry of Agriculture, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2006-05-16 Published:2006-10-28

摘要: 以中国春缺体-四体系为材料,提取各材料的基因组DNA,分别经限制性内切酶EcoRIKpnI完全酶切、电泳、转膜后,用α-32P_dCTP标记的与小麦耐盐相关的糖原合成酶激酶基因TaGSK1为探针进行杂交,结合生物信息学的方法,将该基因定位于普通小麦第一同源群染色体的短臂上,为进一步研究小麦耐盐性的遗传及机理奠定了基础。

关键词: 染色体定位, TaGSK1基因

Abstract: In this paper,Chinese Spring null-tetrasomic lines was studied as material.Their genomic DNA were extrated and were digested completely by EcoRI and KpnI.After electrophoresis and transmembrane,digested genomic DNA hybrided with the probe of the gene of glycogen synthase kinase 1 in Triticum aestivum(TaGSK1),involved in salt-tolerance and marked with α-~(32)P-dCTP.Associated with Bio-information,TaGSK1 was located in the short arm of 1st chromosom group,ie.lAS,1BS and 1DS.This paper established the base of heredity and mechanism in wheat salt-tolerance.

Key words: Chromospmal location, TaGSK1 gene

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引用本文

李亚青, 毛新国, 赵宝存, 葛荣朝, 沈银柱, 黄占景. 小麦糖原合成酶激酶基因(TaGSK1)的染色体定位[J]. 华北农学报, 2006, 21(5): 39-41. doi: 10.3321/j.issn:1000-7091.2006.05.010.

LI Ya-qing, MAO Xin-guo, ZHAO Bao-cun, GE Rong-chao, SHEN Yin-zhu, HUANG Zhan-jing. The Chromosomal Location of Triticum aestivum Glycogen Synthase Kinase Gene (TaGSK1)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(5): 39-41. doi: 10.3321/j.issn:1000-7091.2006.05.010.

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