摘要： 以中国春-HOPE染色体代换系为试验材料,利用细胞膜热稳定性和大田生产条件下高温胁迫两种方法,对与耐热性有关的基因进行了染色体定位。结果表明,无论是利用膜热稳定性方法,还是利用大田高温胁迫环境进行耐热性评价的方法,HOPE的2A,3A,2B,3B和4B染色体代换到中国春相应染色体后,均显著提高了中国春的耐热性,证明这些染色体含有与耐热性有关的基因。在大田高温胁迫环境条件下,HOPE的3D,4A,5A和5B染色体也表现与耐热性有关。利用单染色体代换系和不同耐热性代换系杂交组合,对耐热性的基因效应分析结果显示,HOPE 2A染色体上的耐热性基因表现为显性效应,3A,2B,3A和4B染色体上的耐热性基因表现为加性效应;3B与4B,3A与2A,3B与2B以及这些染色体上的耐热性基因与2A染色体上的耐热性基因之间均可能存在互作效应。
关键词:
小麦,
耐热性,
基因,
染色体定位,
遗传效应
Abstract: With the MT test and method of heat tolerance under field conditions,chromosome substitution lines between Chinese Spring and Hope were used to locate the genes controlling heat tolerance in moderately heat resistant wheat cultivar.The results indicated that chromosomes 2A,3A,2B,3B and 4B of Hope can significantly increases heat tolerance,after substituting correspondent chromosome in Chinese Spring.The investigation of gene effect using hybrid between chromosome substitution lines and Chinese Spring and between chromosome substitution lines with different heat tolerance indicated that the gene(or genes) on Hope chromosome 2A exhibited dominant gene action,and the gene on chromosome 3A,2B.3B and 4B had additive gene action,there may exist gene interaction between genes on chromosome 3B and 4B,3A and 2A,3B and 2B,and between genes on these chromosome and the gene(or genes) on chromosome 2A.
Key words:
Wheat,
Heat tolerance,
Genes,
Chromosome location,
Genet ic effect
中图分类号:
陈希勇, 赵爱菊, 李亚军, 刘玉萍. 小麦耐热性基因的染色体定位和遗传效应分析[J]. 华北农学报, 2007, 22(S2): 1-5. doi: 10.7668/hbnxb.2007.S2.001.
CHEN Xi-yong, ZHAO Ai-ju, LI Ya-jun, LIU Yu-ping. Location of Heat Tolerance Genes on Chromosomes and Analysis of Gene Effect of Heat Tolerance in Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(S2): 1-5. doi: 10.7668/hbnxb.2007.S2.001.