含苏云金芽孢杆菌<em>aiiA</em>基因的重组荧光假单胞菌的构建及对软腐病的抗病活性

doi:10.3321/j.issn:1000-7091.2006.04.004

华北农学报 ›› 2006, Vol. 21 ›› Issue (4): 13-16. doi: 10.3321/j.issn:1000-7091.2006.04.004

所属专题： 萝卜；植物保护；生物技术；

含苏云金芽孢杆菌aiiA基因的重组荧光假单胞菌的构建及对软腐病的抗病活性

张霞^1,3, 胡玉琴², 贾振华¹, 张杰², 马宏¹, 宋水山¹

1. 河北省生物研究所, 河北石家庄 050051;
2. 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室, 北京 100094;
3. 河北农业大学河北保定 071001

收稿日期:2006-05-25 出版日期:2006-08-28
作者简介:张霞（1978- ），女，河北高邑人，博士，主要从事微生物分子生物技术研究。
基金资助:
河北省自然基金资助项目（303610）

Construction of Genetic Modified Pseudomonas fluorescens Which Habouring aiiA Gene from Bacillus thuringiensis and Its Activity Against Soft Rot Disease

ZHANG Xia^1,3, HU Yu-qin², JIA Zhen-hua¹, ZHANG Jie², MA Hong¹, SONG Shui-shan¹

1. Biological Institute of Hebei, Shijiazhuang 050051, China;
2. State Key Lab for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, CAAS, Beijing 100094, China;
3. Agricultural University of Hebei, Baoding 071001, China

Received:2006-05-25 Published:2006-08-28

摘要/Abstract

摘要： aiiA基因编码的aiiA蛋白能降解细菌群体感应系统中的信号分子N_酰基高丝氨酸内酯AHLs，从而减弱致病菌对植物的危害。本文将aiiA基因转入到植物根际促生细菌荧光假单胞菌P303中，构建成防治植物细菌和真菌病害的工程菌P303-ss10。通过PCR和RT-PCR鉴定，均获得0.8 kb的目的片断，表明aiiA基因已经导入P303中。室内平板抑菌试验表明，该工程菌保留了出发菌株对多种植物病原真菌的抑制作用；室内离体试验表明该工程菌对马铃薯软腐病和大白菜软腐病都有一定的抑制作用。

关键词: 荧光假单胞菌工程菌, 群体感应, aiiA基因, 胡萝卜软腐欧文氏杆菌, 细菌病害防治

Abstract: aiiA protein,encoded by aiiA gene,can inactivates the AHL signals which regulates in quorum sensing system in bacteria by hydrolyzing its lactone bond so that it can release the disease in plant.Engineered strain P303-ss10 which can control bacterial and fungi diseases was constructed in this paper by transforming aiiA gene into Pseudomonas fluorescens P303.DNA fragments in 0.8 kb size were obtained by PCR and RT-PCR and it indicated that the engineered strain P303-ss10 was harbouring aiiA gene.P303-ss10 not only remained strong antifungal activity against several kinds of plant pathogenic fungi as P303,but also could control soft rot diseases in potato and Chinese cabbage effectively in vivo.

Key words: Engineered Pseudomonas fluorescens, Quroum sensing, aiiA gene, Erwinia.carotovora pv.carotovora, Bacterial disease control

中图分类号:

张霞, 胡玉琴, 贾振华, 张杰, 马宏, 宋水山. 含苏云金芽孢杆菌aiiA基因的重组荧光假单胞菌的构建及对软腐病的抗病活性[J]. 华北农学报, 2006, 21(4): 13-16. doi: 10.3321/j.issn:1000-7091.2006.04.004.

ZHANG Xia, HU Yu-qin, JIA Zhen-hua, ZHANG Jie, MA Hong, SONG Shui-shan. Construction of Genetic Modified Pseudomonas fluorescens Which Habouring aiiA Gene from Bacillus thuringiensis and Its Activity Against Soft Rot Disease[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(4): 13-16. doi: 10.3321/j.issn:1000-7091.2006.04.004.

http://www.hbnxb.net/CN/Y2006/V21/I4/13

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含苏云金芽孢杆菌aiiA基因的重组荧光假单胞菌的构建及对软腐病的抗病活性

Construction of Genetic Modified Pseudomonas fluorescens Which Habouring aiiA Gene from Bacillus thuringiensis and Its Activity Against Soft Rot Disease

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