摘要： 为进一步探明三河马遗传分化，参考牛（M57764）、羊（AF002110）、猪（M17704）、人（J03071）和鼠（X12630）等哺乳动物GH基因序列，设计一对特异引物，用PCR方法从三河马基因组中扩增出一条长约2kb的DNA片段。PCR产物经pGEM-Teasy载体转化感受态DH5α株大肠杆菌，获得重组克隆子。DNA测序表明：三河马生长激素基因DNA序列长1923bp，含完整的5个外显子和4个内含子，与猪、狗、牛、羊的GH的cDNA序列同源性分别为94.47%，92.63%，90.06%和90.37%。其cDNA所编码氨基酸与猪、狗、牛、羊同源性分别为97.21%，96.74%，88.84%和88.37%，表明该基因在进化过程中是保守的。

关键词: 三河马, GH基因, 克隆, 序列分析

Abstract: Primers designed according to published literatures and the gene sequences of other mammals were used to amplify the full sequence of GH gene for the first time.The results showed,the full length of GH gene with 1923 bp consist of 5 exon,4 intron.The comparison of these sequences with those of other animals through Blastn of GenBank,the results indicated that the identities of cDNA sequence of the GH gene were 94.47%,92.63%,90.06% and 90.37% by homologous comparison among Sanhe horse and other four species(pig,dog,cow and sheep),and that were 97.21%,96.74%,88.84% and 88.37% in amino acid sequences.The results showed that the GH gene was conservative in the process of evolution.

Key words: Sanhe horse, Growth hormone gene, Cloning, Sequence analysis

中图分类号: 

• S821.82

• Q523.8