华北农学报 ›› 2006, Vol. 21 ›› Issue (3): 95-99. doi: 10.3321/j.issn:1000-7091.2006.03.022

所属专题: 黄瓜

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黄瓜霜霉病生物测定技术研究

郝永娟, 王万立, 刘耕春, 刘春艳, 王勇   

  1. 天津市植物保护研究所, 天津 300112
  • 收稿日期:2005-04-30 出版日期:2006-06-28
  • 作者简介:郝永娟(1971- ),女,天津宝坻人,副研究员,主要从事植物病害的防治及杀菌剂应用技术研究工作。
  • 基金资助:
    天津市农业科学院院长基金项目

Study on Bioassay Methods of Cucumber Downy Mildew

HAO Yong-juan, WANG Wan-li, LIU Geng-chun, LIU Chun-yan, WANG Yong   

  1. Tianjin Plant Protection Institute, Tianjin 300112, China
  • Received:2005-04-30 Published:2006-06-28

摘要: 研究了诱发黄瓜霜霉病孢子囊大量产生的条件;明确了黄瓜霜霉病菌可在低温冰冻条件下保存。接种后20℃左右,保持24h黑暗,相对湿度90%以上,以后光照16 h/d,适宜霜霉病菌的侵染发展。比较6种生测方法,以叶盘蘸药漂浮法的敏感性及稳定性最好。

关键词: 黄瓜, 霜霉病, 发病条件, 生测方法

Abstract: In this paper,the condition of sporangium production of Pseudoperonospora cubensis has been discussed.Cucumber leaf with sporangium can be preserved in low temperature and freezing condition.The optimum temperature of infection is around 20℃.Pseudoperonospora cubensis infects with 16 h/d lighting after keeping darkness and RH over 90% for 24 hours.Comparing six bioassay methods for Pseudoperonospora cubensis,the results illustrated that leaf disk with fungicide floating was better than others in sensitivity and stability aspects.

Key words: Cucumber, Downy mildew, Occurrence condition, Bioassay method

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引用本文

郝永娟, 王万立, 刘耕春, 刘春艳, 王勇. 黄瓜霜霉病生物测定技术研究[J]. 华北农学报, 2006, 21(3): 95-99. doi: 10.3321/j.issn:1000-7091.2006.03.022.

HAO Yong-juan, WANG Wan-li, LIU Geng-chun, LIU Chun-yan, WANG Yong. Study on Bioassay Methods of Cucumber Downy Mildew[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(3): 95-99. doi: 10.3321/j.issn:1000-7091.2006.03.022.

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