摘要： 以拟南芥基因组DNA为模板, 通过PCR扩增得到逆境胁迫诱导表达基因rd29A的启动子片段, 将其克隆到pUC19质粒中进行序列分析.结果表明, 获得的启动子片段大小为97 bp, 与已报道的该启动子序列比较, 其核苷酸序列同源性为99.8%.
关键词:
拟南芥,
rd29A,
启动子,
抗逆基因
Abstract: The promoter of the rd29A gene was amplified from Arabidopsis thaliana(Columbia ecotype)genome by polymerase chain reaction and cloned into pUC19 vector. Sequence analysis showed that the obtained promoter fragment contained 937 nucleotides and showed sequence homology of 99.8% with the reported rd29A promoter.
Key words:
Arabidopsis thaliana,
rd29A,
Promoter,
Stress-inducible,
gene
中图分类号:
吴梅花, 张丽, 牛一丁, 方天祺, 哈斯阿古拉. 拟南芥逆境胁迫诱导表达基因rd29A启动子的克隆与序列分析[J]. 华北农学报, 2005, 20(2): 5-7. doi: 10.3321/j.issn:1000-7091.2005.02.002.
WU Mei-hua, ZHANG Li, NIU Yi-ding, FANG Tian-qi, Hasi Agula. Cloning and Sequencing of the Promoter of Stress-inducible Gene rd29A from Arabidopsis thaliana[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2005, 20(2): 5-7. doi: 10.3321/j.issn:1000-7091.2005.02.002.