华北农学报 ›› 2005, Vol. 20 ›› Issue (2): 19-22. doi: 10.3321/j.issn:1000-7091.2005.02.006

所属专题: 白菜

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酚类化合物对大白菜遗传转化效率的影响

赵军良1, 逯保德2, 徐鸿林3, 朱祯3, 赵美华2, 梁爱华4   

  1. 1. 山西大学, 生物技术研究所, 化学生物学与分子工程教育部重点实验室, 山西, 太原, 030006;
    2. 山西省农业科学院蔬菜研究所, 山西, 太原, 030031;
    3. 中国科学院遗传研究所, 北京, 100101;
    4. 山西大学, 生物技术研究所, 化学生物学与分子工程教育部重点实验室, 山西, 太原, 030006
  • 收稿日期:2004-11-09 出版日期:2005-04-28
  • 作者简介:赵军良(1963-),男,山西昔阳人,副研究员,主要从事蔬菜遗传转化和分子生物学研究工作.
  • 基金资助:
    山西省回国留学人员基金资助项目(2000-10);山西省农业科学院高新技术基金资助项目(02YGX04)

Effect of Phenolic Compounds on Efficiency of Genetic Transformation in Chinese Cabbage

ZHAO Jun-liang1, LU Bade2, XU Hong-lin3, ZHU Zhen3, ZHAO Mei-hua2, LIAN GAi-hua4   

  1. 1. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biological Technology, Shanxi University, Taiyuan 030006, China;
    2. Institute of Vegetables, Shanxi Academy of Agricultural Science, Taiyuan 030031, China;
    3. Institute of Genetics, Chinese Acacemy of Science, Beijing 100101, China
  • Received:2004-11-09 Published:2005-04-28

摘要: 以大白菜纯合系河头早B为试验材料, 用携带质粒pBinΩSCK的根癌农杆菌(Agrobacterium tumefaciens)LBA0转化其无菌苗子叶, 比较了8种酚类化合物对大白菜遗传转化效率的影响.结果表明, 乙酰丁香酮和邻苯二酚对外植体的转化频率影响最大;阿魏酸和ρ-羟基苯甲酸的作用次之;芥子酸和焦性没食子酸有一定的促进作用;香草醛和丁香酸没有明显的作用.复合酚类化合物的促进作用优于单一酚类化合物的作用.

关键词: 酚类化合物, 遗传转化, 子叶

Abstract: A grobacterium strain LBA 4404 carrying pBinΩSCK was used to t ransfer the cotyledons of asceptic plant s of Chinese cabbage. Effect of 8 kinds of phenolic compounds on the efficiency of genetic transformation was compared. The result s indicated that the effect of acetonesyringone and brenzcatechin were the the most obvious. Transferulic acid and phydroxybenzoic acid took the second place. Erucic acid and pyrogallic acid had some effect. Venillin and 42hydroxy23, 52dimethoxy benzoesaure had no clear effect. The promoting effect of multiphenolic compounds superior to single phenolic compound.

Key words: Phenolic compounds, Genetict ransformation, Cotyledon

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引用本文

赵军良, 逯保德, 徐鸿林, 朱祯, 赵美华, 梁爱华. 酚类化合物对大白菜遗传转化效率的影响[J]. 华北农学报, 2005, 20(2): 19-22. doi: 10.3321/j.issn:1000-7091.2005.02.006.

ZHAO Jun-liang, LU Bade, XU Hong-lin, ZHU Zhen, ZHAO Mei-hua, LIAN GAi-hua. Effect of Phenolic Compounds on Efficiency of Genetic Transformation in Chinese Cabbage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2005, 20(2): 19-22. doi: 10.3321/j.issn:1000-7091.2005.02.006.

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