华北农学报 ›› 1998, Vol. 13 ›› Issue (1): 71-73. doi: 10.3321/j.issn:1000-7091.1998.01.014

• 研究简报 • 上一篇    下一篇

辐射诱变甜荞高产突变体过氧化物酶活性及其同工酶的研究

张小冰1, 郝建平2, 裴雁曦2, 李国柱3, 刘晓琴2   

  1. 1. 太原师范专科学校生化系;
    2. 山西大学生命科学系;
    3. 山西农业大学农业生物物理室
  • 收稿日期:1997-06-20 出版日期:1998-03-28
  • 基金资助:
    山西省科委资助

Study on Peroxidase Activity and Its Isoenzymes of High yield Mutant in Radiation Breeding of Common Buckwheat

Zhang Xiaobing1, Hao Jianping2, Pei Yanxi2, Li Guozhu3, Liu Xiaoqin2   

  1. 1. Biology and Chemistry Department,Tai yuan Teachers’College,Taiyuan 030001;
    2. Life Science Department,Shanxi University,Taiyuan;
    3. Research Room of Biophysics,Shanxi Agricualtural University,Taigu
  • Received:1997-06-20 Published:1998-03-28

摘要: 甜荞种子经60Co-γ射线照射后从10000rad的剂量组中选出了两个优良高产突变体Ⅱ-45-1和Ⅲ-36-1.研究表明,Ⅱ-45-1、Ⅲ-36-1的过氧化物酶活性均比对照(未经辐照处理的甜荞种子)高2倍。过氧化物酶同工酶酶谱显示,Ⅱ-45-1和Ⅲ-36-1均比对照增加一条Rf值为0.43的酶带,减少一条Rf值为0.84的酶带。

关键词: 甜荞, 突变体, 过氧化物酶, 过氧化物酶同工酶

Abstract: Two fine high yielding mutants (Ⅱ-45-1 and Ⅲ-36-1)of common buckwheat were obtained from 10 000 rad seed radiation by 60 Co γ ray.The results indicates that the peroxidase activity of Ⅱ 45 1 and Ⅲ 36 1 is three times that of unradiated buckwheat.The isoenzymes zymogram of peroxidase shows that both Ⅱ-45-1 and Ⅲ-36-1 have an additional band (Rf=0.43)while a band (Rf=0.84)is missing in comparison with the unradiated buckwheat.

Key words: Common buckwheat, Mutant Peroxidase, Isoenzyme of peroxidase

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引用本文

张小冰, 郝建平, 裴雁曦, 李国柱, 刘晓琴. 辐射诱变甜荞高产突变体过氧化物酶活性及其同工酶的研究[J]. 华北农学报, 1998, 13(1): 71-73. doi: 10.3321/j.issn:1000-7091.1998.01.014.

Zhang Xiaobing, Hao Jianping, Pei Yanxi, Li Guozhu, Liu Xiaoqin. Study on Peroxidase Activity and Its Isoenzymes of High yield Mutant in Radiation Breeding of Common Buckwheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1998, 13(1): 71-73. doi: 10.3321/j.issn:1000-7091.1998.01.014.

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