摘要： 由于雄性配子（精子）有性染色体的差异，哺乳动物X、Y精子在质量或密度上存在着微小的差别。为了突出这微小的质量差，实验先将两性精子共有的顶体和尾部都去掉，然后再将精子细胞核在氯化铯密度梯度条件下离心，成功地将乳牛精子细胞核分成明显的两个区带。

关键词: 乳牛, X-精子, Y-精子, 细胞核, 分离

Abstract: Owing to the difference of sexual chromosomes of mammalian male gametes (sperm),X- and Y- bearing sperm were tiny difference in quality or densityTo give prominence to the quality difference between X- and Y- bearing sperm at first,the acrosomes and tails were taken off,then the sperm muclei were centrifuged in the density gradient medium of cesium chloride,and they were separated into two fractions successfully.

Key words: X-sperm, Y-sperm, Sperm nuclei, Density gradient centrifugation

中图分类号: 

• S823.2