摘要： 采用山西栽培的5个地方名蒜茎尖离体培养，获得了无病毒蒜种。结果表明，茎尖大小为0.2～0.5mm，不定芽增殖培养基以B5或MS.附加1～2mg/L细胞分裂素和0.01～0.1mg/LNAA，效果最佳。根系诱导在原培养基中加入0.2mg/LNAA，出根率可达90%以上。成苗后于12月份移栽到节能日光温室，成活率达90%以上。经对脱毒苗形态观察和染色体检测，没有发生异常变化，植株生长健壮，遗传性稳定，产量高。

关键词: 大蒜, 茎尖培养, 脱毒, 愈伤组织

Abstract: Virus-free garlics were obtained by cultrring stem-tips of 5 of 5 famous local garlics cultivated in Shanxi. The results showed ththat when the media were MS or B5 supplemented with 1 -2mg/L BA and 0. 01 -0. 1ing/L NAA an,andehof zstem-tip eas 0. 2-0. 5mm, increase of regenerated buds was the most, and that the rratio of the induction of roots was more than 90% when the medium was MS or B5Zcontaining.02mg/L NAA, T. Tsurvival tate was 90% or above after the regenerated plants were transplanted into the energy-saving daylight greenhouse.The results also proved that the virus-free garlic seedlings had not any abnormal changes. They could grow robustly and had a steady heredity and high production through investigating their shapes and examining their chromosomes.

Key words: Garlic, Stem-tip culture, Virus-free, Callus

中图分类号: 

• S633.401