华北农学报 ›› 1993, Vol. 8 ›› Issue (3): 23-27. doi: 10.3321/j.issn:1000-7091.1993.03.005

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几个家蚕品种不同性别血淋巴酯酶同工酶的比较

蔡宏玉, 张晓义   

  1. 河北省农林科学院蚕桑研究所,承德 067000
  • 收稿日期:1992-09-25 出版日期:1993-09-28

Comparisoin on Hemolymph Esterase Isozyme of Silkworm Strains with Different Sex

Cai Hongyu, Zhang Xiaoyi   

  1. Silkworm and Mulberry Institute, Hcdei Academy of Agricultural and Forestry Sciences, Chengde
  • Received:1992-09-25 Published:1993-09-28

摘要: 用聚丙烯酰胺等电聚焦电泳,分析了不同血统和化性的九个品种的家蚕(Bombyxmori)血淋巴酯酶同工酶(EST)。结果表明,所有的品种五龄盛食期幼虫都呈现相似的酶谱,可区分为四个区带(ESTⅠ、Ⅱ、Ⅲ和Ⅳ),共出现18条带,带的数目和酶活强度随品种而异。在五个品种中的Ⅲ-3及四个品种中的Ⅱ-2出现了伴性现象,Ⅲ-3伴随雄性、Ⅱ-2伴随雌性出现。

关键词: 家蚕, 性别, 酯酶, 同工酶

Abstract: Using isoclcctric focusing elcctrophoresis with olyactylamide, we analyzed the hcmolymph csterasc zymograms with nine silkworm strains of different descent and generation number.The result shows that all of the strains detect similar zymogram on the forth day after the post-molt feeding of the fifth inslar.We observed eighteen isozymc band all together which can be dcvide into four regions(EST Ⅰ,Ⅱ,Ⅲ,Ⅳ).The numbers and activities of isozymc bands are different according to the strains.The Ⅲ-3 band of five strains and the Ⅱ-2 band of four strains appear the sex-lankcd phenomenon.The Ⅲ-3 band appears male phenomenon and the Ⅱ -2 band with female phenomenon.

Key words: Silkworm, Sex, Hcmolymph, Estcrase isozyme

引用本文

蔡宏玉, 张晓义. 几个家蚕品种不同性别血淋巴酯酶同工酶的比较[J]. 华北农学报, 1993, 8(3): 23-27. doi: 10.3321/j.issn:1000-7091.1993.03.005.

Cai Hongyu, Zhang Xiaoyi. Comparisoin on Hemolymph Esterase Isozyme of Silkworm Strains with Different Sex[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1993, 8(3): 23-27. doi: 10.3321/j.issn:1000-7091.1993.03.005.

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