摘要： 牦牛与普通牛的种间杂种犏牛雄性不育机理一直是畜牧科学研究的热点之一，对牦牛、犏牛睾丸组织特异表达基因的比对分析，可为犏牛雄性不育分子调控机制提供基因参考。通过对牦牛及杂种犏牛TB-RBP基因进行克隆，并利用实时荧光定量PCR技术对候选基因进行组织表达差异分析。结果表明，克隆获得牦牛TB-RBP基因CDS全序列873 bp，犏牛TB-RBP基因部分CDS区序列587 bp；系统进化树显示不同物种TB-RBP基因编码区序列高度保守，遗传相似性较高；蛋白功能预测TB-RBP蛋白属于Translin结合蛋白家族，对精子发生等生物过程具有重要调控作用。TB-RBP基因在犏牛和牦牛的睾丸组织中均有表达， TB-RBP基因的表达水平在牦牛与犏牛组间差异显著（0.01 < P < 0.05），牦牛显著高于犏牛。TB-RBP基因是精子正常发育的关键基因，而在犏牛睾丸组织中表达量较低，表明犏牛雄性不育与精子发生异常有关，为今后开展TB-RBP基因与犏牛雄性不育的相关分析以及基因定位、表达调控和牦牛分子育种奠定了理论基础。

关键词: 犏牛, 牦牛, TB-RBP基因, 睾丸组织, 组织表达

Abstract: The mechanism of male sterility of hybridization of yak with ordinary cattle of other species is one of the hot topics to researchers. Through the comparative analysis of the specific expression genes in yak and yak-cattle testis,it is expected to provide a genetic reference for the regulation mechanism of male sterility in yak-cattle. It was detected to cloning and expression of TB-RBP genes in testis of yak and yak-cattle by quantitative RT-PCR. The results showed that CDS sequence of TB-RBP gene was 873 bp in yak,and partial CDS sequence was 587 bp in yak-cattle. The phylogenetic tree showed that the coding region of the TB-RBP gene of different species was highly conserved and high genetic similarity. Protein function prediction showed that TB-RBP protein belonged to the translin binding protein family,which was played an important role in regulating biological processes such as spermatogenesis. TB-RBP mRNA expressed in these nine tissues. The difference expression of TB-RBP mRNA in yak was higher than yak-cattle(0.01 < P < 0.05). TB-RBP gene was key gene for normal sperm development,and the expression level was lower in testicular tissue of yak-cattle. It indicated that male infertility in yak-cattle was associated with abnormal spermatogenesis. The results establish the base of farther research in molecular level. It may be helpful to offering theory gist in compare research between yak and yak-cattle.

Key words: Yak-cattle, Yak, TB-RBP gene, Testicular tissue, Tissue expression

中图分类号: 

• S823

• S813

• Q78