大豆<i>GmNAC23</i>基因的克隆及特征分析

doi:10.7668/hbnxb.201751084

摘要/Abstract

摘要： NAC是一类植物特异性转录因子，其在调节非生物和生物胁迫的发育和响应中起重要作用。为了探索大豆相关基因的功能，利用PCR的方法从大豆中克隆了1个NAC基因GmNAC23。该基因cDNA全长1 077 bp，开放阅读框长1 053 bp，编码350个氨基酸，蛋白质分子量为39.483 ku，等电点为7.08。序列分析表明，GmNAC23基因组包含3个外显子和2个内含子。多序列比对结果表明，GmNAC23蛋白含有1个高度保守的NAM结构域。进化树分析表明，该蛋白与绿豆、木豆、鹰嘴豆的NAC蛋白具有同源关系。转录活性分析结果表明，GmNAC23在SD（Trp-/His-/Ade-）营养型缺陷培养基上生长，说明该基因在酵母体内具有转录激活功能。组织特异性表达模式分析表明，在检测的所有组织中GmNAC23都有表达，在根中表达量最高，在茎顶端分生组织中表达量最低。结果表明，大豆GmNAC23基因具有转录激活功能，并且其在根中大量表达。为进一步研究GmNAC23的生物学功能奠定了基础。

关键词: 大豆, GmNAC23, 克隆, 转录激活

Abstract: NAC is a plant-specific transcription factor that plays an important role in regulating the development and response of abiotic and biotic stresses. In order to explore the function of soybean-related genes, a NAC gene GmNAC23 was cloned from soybean by PCR. The full length of GmNAC23 cDNA was 1 077 bp, including an ORF of 1 053 bp. This cDNA sequence encoded a polypepide of 350 amino acid residues with a predicted molecular mass of 39.483 ku and a basic isoelectric point of 7.08. Sequence analysis showed that the GmNAC23 genome contained three exons and two introns. Multiple sequence alignments showed that the GmNAC23 protein contained a highly conserved NAM domain. Phylogenetic analysis showed that the protein had the homology with the NAC protein of VrNAC, CaNAC and CarNAC. Transcriptional activation ability assay showed that the GmNAC23 could grow on SD (Trp-/His-/Ade-) deficient medium, indicating that the gene has transcriptional activation function in yeast. Tissue-specific expression profiles showed that GmNAC23 expressed almost in all detected tissues, and its expression level was relatively high in root and low in sam.The results indicated that the soybean GmNAC23 gene had a transcriptional activation function and was abundantly expressed in root, which laid a foundation for further study of the biological function of GmNAC23.

Key words: Soybean, GmNAC23, Cloning, Transcriptional activation

中图分类号:

Q78
S565.03

王萍, 于月华, 白玉翠, 郭坤鹏, 康嘉楠, 倪志勇. 大豆GmNAC23基因的克隆及特征分析[J]. 华北农学报, 2019, 34(1): 46-53. doi: 10.7668/hbnxb.201751084.

WANG Ping, YU Yuehua, BAI Yucui, GUO Kunpeng, KANG Jianan, NI Zhiyong. Cloning and Characterization of Soybean GmNAC23 Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(1): 46-53. doi: 10.7668/hbnxb.201751084.

http://www.hbnxb.net/CN/Y2019/V34/I1/46

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