摘要： 为研究四川猪场分离的耐甲氧西林金黄色葡萄球菌（MRSA）的耐药现状及其SCCmec基因型情况，讨论猪源MRSA对公共卫生带来的隐患。于2013年3-12月在四川地区12个规模化猪场采集的120份样品中分离金黄色葡萄球菌（SA），对PCR初步鉴定出SA，再鉴定mecA、femA耐药基因，阳性菌株判定为MRSA。对鉴定出的猪源MRSA进行SCCmec分型与耐药性试验及分析。共分离出60株金黄色葡萄球菌，其中22株MRSA，检出率18.3%（22/120），饲养员手棉拭子、猪鼻捻子、猪场水样中均分离出MRSA。药敏试验结果表明，22株MRSA菌株的多重耐药性明显，耐药性主要表现为4、6耐，其中SCCmec Ⅳ a型17株、SCCmecⅠ型5株。目前四川地区猪源MRSA不仅对猪场人员存在感染隐患，同时也对公共卫生安全存在一定的威胁。

关键词: 耐甲氧西林金黄色葡萄球菌, SCCmec分型, 耐药性, 公共卫生

Abstract: To study the drug resistance of SCCmec resistant Staphylococcus aureus and analyze pubic health in Sichuan,we collected 120 samples from Sichuan pig-farms to identify MRSA by PCR test from Sichuan. Then identified these 22 MRSA by drug-resistance testing and SCCmec typing.In these samples,60 strains of Staphylococcus aureus had been isolated from pig water and the hand of worker,including 22 MRSA,the detection rate was 18.3% (22/120). Susceptibility testing results showed that this 22 multi-drug resistant MRSA strains had significant multi-drug resistance,most were 4,6-resistant. Genotyping results showed that the predominant type of the Sichuan MRSA were mainly SCCmec Ⅳ a type,including 17 strains of SCCmec Ⅳ a type and 5 strains of SCCmec Ⅰ type.Last discuss the MRSA from swine have imperiled the health of worker and public health。

Key words: Methicillin-resistantst Staphylococcus aureus, SCCmec, Typing drug sensitivity test, Public health

中图分类号: 

• Q78

• S442