摘要： 为了解浙江省规模化猪场患病猪副猪嗜血杆菌的耐药性,采用K-B琼脂法对73株HPS浙江株进行了18种临床药物的敏感性试验,同时应用PCR技术对11个相关耐药基因进行扩增、测序和比对分析。药敏试验结果显示:对磺胺类耐药的分离株比例高达86.30%,对四环素类、氨基糖苷类、喹诺酮类、β-内酰胺类和氯霉素类耐药的比例分别为65.75%,60.27%,50.68%,50.68%,46.58%,对大环内酯类耐药仅为28.77%。PCR分析显示:97.26%的菌株中检测到耐药基因,多重耐药的占84.93%。其中喹诺酮类耐药基因Aac(6’)-1b的检出率为76.71%,氨基糖苷类耐药基因AadA1的检出率为65.75%,磺胺类耐药基因Sul1和Sul2的检出率为45.2%和10.96%,氯霉素类耐药基因Flor、CmLA、Cat1的检出率为15.07%,8.22%,58.9%,四环素类耐药基因TetA和TetB的检出率为4.11%和49.32%,β-内酰胺类耐药基因Tem-11的检出率为35.62%,大环内酯类耐药基因ErmB的检出率为1.37%。耐药基因型和表型符合率为:磺胺类62.07%,β-内酰胺类74.29%,氯霉素类81.48%,四环素类75.00%,大环内酯类0,喹诺酮类75.68%,氨基糖苷类72.09%。因此,HPS在浙江规模化猪场对临床药物已产生了耐药性,通过耐药基因检测结果确定耐药菌株具有一定的参考价值。
关键词:
副猪嗜血杆菌,
耐药性,
耐药基因
Abstract: This study was conducted to investigate the resistance to common antibiotics and genes of Haemophi- lus parasuis isolated from the scaled pig farms in Zhejiang province．A total of 73 Zhejiang H．parasuis isolates were tested for their resistance to 18 antibiotics by K- B method．PCR was used to detect related genes．The amplified products were sequenced and aligned．The results showed that 86．30% isolates were resistant to sulfonamides， 65.75%，60．27%，50．68%，50．68% and 46．58% isolates were resistant to tetracyclines，aminoglycosides，quino- lones，- lactam antibiotics，chloramphenicols，respectively，while only 28．77% isolates were resistant to macrolides． Resistance genes were detected in 97．26% isolates，and multiple resistance genes were found in 84．93% isolates． The rates of resistance genes Aac(6')－ 1b，AadA1，Sul1，Sul2，Flor，CmLA，Cat1，TetA，TetB，Tem- 11 and ErmB were 76．71%，65．75%，45．2%，10．96%，15．07%，8．22%，58．9%，4．11%，49．32%，35．62% and 1．37%，respec- tively．The coincidence of phenotype and genotype for the isolates to resist sulfonamides，- lactam antibiotics，chlor- amphenicols，tetracyclines，macrolides，quinolones and aminoglycoside antibiotics were 62．07%，74．29%， 81. 48%，75．00%，0，75．68% and 72．09%，respectively．Therefore，H．parasuis has developed resistance to clini- cally commonly used antibiotics and the results from analysis of drug- resistant gene could be used as reference to determine resistance bacteria．
Key words:
Haemophilus parasuis,
Drug resistance,
Drug- resistant gene
中图分类号:
忽占利, 李军星, 胡松华, 王一成, 袁秀芳, 徐丽华. 副猪嗜血杆菌分离株的耐药性及耐药基因分析[J]. 华北农学报, 2013, 28(4): 228-233. doi: 10.3969/j.issn.1000-7091.2013.04.042.
HU Zhan-li, LI Jun-xing, HU Song-hua, WANG Yi-cheng, YUAN Xiu-fang, XU Li-hua. Phenotype and Genotype Analysis of Antibiotic Resistance of Haemophilus parasuis Isolated from the Scaled Pig Farms in Zhejiang Province[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(4): 228-233. doi: 10.3969/j.issn.1000-7091.2013.04.042.