摘要： 2C型丝氨酸/苏氨酸蛋白磷酸酶（PP2C-type protein phosphatases，PP2C）是ABA信号转导途径中的关键组分，在植物生长发育、细胞周期调节及应对逆境胁迫的过程中发挥重要的作用。为探究PP2C基因在烟草适应非生物胁迫中的功能，从烟草栽培品种K326中克隆到了一个PP2C同源基因，该基因开放阅读框为1 617 bp，编码538个氨基酸残基。同源性分析结果显示，该基因所编码的蛋白与绒毛状烟草PP2C16的亲缘关系最近，故命名为NtPP2C16。生物信息学分析表明，NtPP2C16催化区域上具有PP2C家族进化中相对保守的11个结构亚区。qRT-PCR研究分析结果表明：该基因的表达显著受ABA和H₂O₂ 2种信号分子诱导，并且响应干旱、高盐、低温和低钾胁迫。成功构建NtPP2C16-pBI121过表达载体，为解析NtPP2C16参与烟草非生物逆境胁迫响应提供一定的理论依据。

关键词: 烟草, NtPP2C16, 克隆, 序列分析, 表达

Abstract: 2C protein phosphatase (PP2C) is a key component in the ABA signal transduction pathway and plays a pivotal role in plant growth and development,cell cycle regulation and adaptation to adversity stresses.To explore the function of the PP2C gene in tobacco adaptation to abiotic stress,a PP2C homologous gene was cloned from the tobacco cultivar K326,which contained a 1 617 bp ORF encoding 538 amino acid. The homology analysis showed that the gene had high homology with PP2C16 of Nicotiana tomentosiformis. So it was named NtPP2C16. Bioinformatics analysis showed that the NtPP2C16 catalytic region had 11 structural sub-regions which were relatively conservative in the PP2C family evolution. qRT-PCR analysis revealed that the expression of this gene was significantly induced by ABA and H₂O₂ signal molecules,and responded to drought,high salt,low temperature and low potassium stresses. NtPP2C16-pBI121 overexpression vector was constructed successfully,the results provided some basis for analysis of NtPP2C16 responsing to abiotic stresses in tobacco.

Key words: Tobacco, NtPP2C16, Cloning, Sequence analysis, Expression

中图分类号: 

• Q78

• S572.03