华北农学报 ›› 2016, Vol. 31 ›› Issue (5): 35-43. doi: 10.7668/hbnxb.2016.05.006

所属专题: 棉花

• 论文 • 上一篇    下一篇

棉花转录因子GhSNAC3启动子的克隆与表达分析

马骏骏1,2, 柳展基1, 李菲1, 王立国1, 傅明川1, 朱新霞2, 刘任重1   

  1. 1. 山东棉花研究中心, 山东 济南 250100;
    2. 石河子大学 生命科学学院, 农业生物技术重点实验室, 新疆 石河子 832003
  • 收稿日期:2016-07-10 出版日期:2016-10-28
  • 通讯作者: 朱新霞(1968-),女,新疆石河子人,副教授,博士,主要从事植物生物技术和分子育种研究;刘任重(1965-),男,山东诸城人,研究员,博士,主要从事棉花转基因育种研究。
  • 作者简介:马骏骏(1989-),女,黑龙江桦南人,在读硕士,主要从事植物逆境分子生物学研究。
  • 基金资助:
    国家自然基金项目(31571755);山东省优秀中青年科学家科研奖励基金计划项目(BS2015NY014);山东省农业科学院青年基金项目(2914QNM28)

The Cloning and Expression Analysis of Transcription Factor GhSNAC3 Promoter in Cotton

MA Junjun1,2, LIU Zhanji1, LI Fei1, WANG Liguo1, FU Mingchuan1, ZHU Xinxia2, LIU Renzhong1   

  1. 1. Shandong Cotton Research Center, Jinan 250100, China;
    2. College of Life Sciences, Shihezi University, Key Laboratory of Agrobiotechnology, Shihezi 832003, China
  • Received:2016-07-10 Published:2016-10-28

摘要: 为研究棉花NAC转录因子基因GhSNAC3启动子的结构和功能,以鲁棉研32号基因组为模板,用PCR扩增的方法得到棉花基因GhSNAC3的启动子序列,利用分析网站Plant CARE、PLACER和BDGP对启动子上存在的顺式作用元件进行了预测与分析,在此基础上利用该启动子及GhSNAC3基因构建植物表达载体,并通过烟草遗传转化进一步研究启动子的转录活性。结果表明GhSNAC3启动子除含有CAAT-box等基本顺式作用元件外,还含有茉莉酸、赤霉素和脱落酸响应元件以及大量光顺式作用元件和逆境胁迫诱导相关的顺式调控元件。采用不同胁迫处理转基因烟草并进行表达分析,结果显示GhSNAC3在盐胁迫下植株根部有高水平的表达,而在茎和叶中表达量极低,表明GhSNAC3在逆境胁迫应答过程中可能具有重要功能,其启动子是一个盐诱导型启动子,具有组织特异性。研究结果为棉花NAC信号通路研究和耐盐基因工程改良提供了理论依据。

关键词: 棉花, NAC, 启动子, 盐诱导

Abstract: In order to analyze the structure and function of the promoter of cotton NAC transcription factor gene GhSNAC3,the promoter fragment was cloned and identified from Lumianyan 32 in Gossypium hirsutum L..The cis-acting elements in the promoter sequence was predicted and analyzed by using the online database of Plant CARE,PLACER and BDGP.A plant expression vector consisting of GhSNAC3 and its promoter was constructed and transformed into Nicotiana tabacum L.to investigate the transcription activities of the promoter under different stress treatments.The results showed that besides the basic cis-acting elements such as CAAT-box,there existed several MeJA,GA and ABA-responsive elements as well as multiple light-responsive elements and stress-induced controlling elements in the promoter.Under salt stress GhSNAC3 had a high level of expression in the roots of transgenic tobacco plants,while the expression level was low in the stems and leaves,suggesting that the gene GhSNAC3 might played an important role in stress responses in cotton,and its promoter might be tissue-specific and salt-inducible.The results provide the basis for the further study of cotton NAC signal pathway as well as salt tolerance improvement through genetic engineering in the future.

Key words: Cotton, NAC, Promoter, Salt-induced

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引用本文

马骏骏, 柳展基, 李菲, 王立国, 傅明川, 朱新霞, 刘任重. 棉花转录因子GhSNAC3启动子的克隆与表达分析[J]. 华北农学报, 2016, 31(5): 35-43. doi: 10.7668/hbnxb.2016.05.006.

MA Junjun, LIU Zhanji, LI Fei, WANG Liguo, FU Mingchuan, ZHU Xinxia, LIU Renzhong. The Cloning and Expression Analysis of Transcription Factor GhSNAC3 Promoter in Cotton[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(5): 35-43. doi: 10.7668/hbnxb.2016.05.006.

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