摘要： 通过研究蓝刺头粗多糖及其洗脱纯化组分蓝刺头多糖A、B、C的体外抗氧化和抗菌作用，初步确定蓝刺头多糖的生物活性。结果显示，添加浓度相同时，蓝刺头粗多糖的总还原能力以及清除DPPH有机自由基能力最强，显著高于蓝刺头多糖A，极显著高于蓝刺头多糖B、C。当添加浓度为0.1～0.3 mg/mL时，蓝刺头粗多糖清除羟基自由基的能力略高于蓝刺头多糖A，但差异不显著；当添加浓度为0.4 mg/mL时，蓝刺头粗多糖清除羟基自由基的能力略高于蓝刺头多糖A，蓝刺头多糖A略高于蓝刺头多糖B，三者差异不显著，但均极显著高于蓝刺头多糖C，当添加浓度为0.5 mg/mL时，蓝刺头粗多糖清除羟基自由基的能力显著高于蓝刺头多糖A、B，极显著高于蓝刺头多糖C。试验条件下，蓝刺头粗多糖和蓝刺头多糖A对大肠杆菌为低度敏感，最低抑菌浓度均为0.62 mg/mL，蓝刺头多糖B和C对大肠杆菌不敏感，最小抑菌浓度均为1.25 mg/mL；蓝刺头粗多糖、蓝刺头多糖A、B和C对金黄色葡萄球菌为不敏感，最低抑菌浓度分别为1.25，2.50，2.50，2.50 mg/mL；蓝刺头粗多糖、蓝刺头多糖B、C对铜绿假单胞菌为不敏感，最低抑菌浓度均为5.00 mg/mL，蓝刺头多糖A对铜绿假单胞菌无抑制作用；蓝刺头粗多糖和蓝刺头多糖A、B、C对沙门氏菌均为不敏感，最低抑菌浓度分别为2.50，2.50，1.25，5.00 mg/mL。说明蓝刺头粗多糖的抗氧化能力最强，其次为蓝刺头多糖A，而蓝刺头多糖B和C的抗氧化能力较弱。蓝刺头粗多糖和蓝刺头多糖A具有一定的抗大肠杆菌活性，蓝刺头粗多糖和蓝刺头多糖A、B、C均无抗金黄色葡萄球菌、铜绿假单胞菌以及沙门氏菌活性。

关键词: 蓝刺头, 多糖, 抗氧化活性, 抗菌活性

Abstract: In this study antioxidant activity and antibacterial activity of Echinops latifolius Tausch polysaccharides was researched.The result showed that when the initial concentrations were same,ETCP has a highest effect of total reducing capacity,clearance of DPPH free radical in vitro,higher than ETPA,significantly higher than ETPB and ETPC.When the added concentrations were 0.1-0.3 mg/mL,ETCP has a little higher scavenging effects on free radicals than that of ETPA,but no obvious difference;when the added concentrations were 0.4 mg/mL,ETCP has a little higher scavenging effects on free radicals than that of ETPA,ETPA has a little higher scavenging effects than that of ETPB,and difference were not significant,they were significantly higher than that ETPC,when the added concentrations were 0.5 mg/mL,ETCP has a higher scavenging effects on free radicals than that of ETPA,ETPB,significantly higher than ETPC.Experimental condition,ETCP and ETPA were less sensitive to E.coli,the minimal inhibitory concentration(MIC) were 0.62 mg/mL, ETPB and ETPC were insensitive the MIC were 1.25 mg/mL; ETCP,ETPA,ETPB and ETPC were insensitive to Staphylococcus aureus,the MIC were 1.25,2.50,2.50,2.50 mg/mL respectively;ETCP,ETPB and ETPC were insensitive to Pseudomonas aeruginosa,the MIC were 5.00 mg/mL, ETPA had no inhibition to Pseudomonas aeruginosa;ETCP,ETPA,ETPB and ETPC were insensitive to Salmonella,the MIC were 2.50,2.50,1.25,5.00 mg/mL respectively.It means ETCP has the strongest antioxidant effect and secondly for ETPA,ETPB and ETPC were weak.ETCP and ETPA has a certain resistance to activity of E.coli, and ETPA,ETPB and ETPC has no antibacterial action to Staphylococcus aureus,Pseudomonas aeruginosa and Salmonella.

Key words: Echinops latifolius Tausch, Polysaccharide, Antioxidant activity, Antibacterial activity

中图分类号: 

• S853.74