甘蓝枯萎病抗性基因<em>FOC1</em>序列分析及抗性相关变异位点分析

doi:10.7668/hbnxb.2016.03.001

华北农学报 ›› 2016, Vol. 31 ›› Issue (3): 1-10. doi: 10.7668/hbnxb.2016.03.001

所属专题： 生物技术；

甘蓝枯萎病抗性基因FOC1序列分析及抗性相关变异位点分析

雷蕾^1,2, 马建², 吕红豪³, 颉建明¹, 郁继华¹, 康俊根²

1. 甘肃农业大学园艺学院, 甘肃兰州 730070;
2. 北京市农林科学院蔬菜研究中心, 农业部华北地区园艺作物生物学与种质创制重点实验室, 北京 100097;
3. 中国农业科学院蔬菜花卉研究所, 北京 100081

收稿日期:2016-03-18 出版日期:2016-06-28
通讯作者: 郁继华(1961-),男,江苏无锡人,教授,博士,主要从事蔬菜栽培生理及生长调控研究;康俊根(1972-),男,山西忻州人,研究员,博士,主要从事蔬菜遗传育种及分子生物学研究。
作者简介:雷蕾(1989-),女,甘肃天水人,在读硕士,主要从事蔬菜分子育种研究。
基金资助:
"十二五"国家科技支撑计划项目(2012BAD02B01;2014BAD01B08);北京市科委项目(Z141105002314020)

Analysis of Sequences and Variation Sites of Fusarium Wilt Resistance Gene FOC1 in Brassica oleracea

LEI Lei^1,2, MA Jian², LV Honghao³, XIE Jianming¹, YU Jihua¹, KANG Jungen²

1. College of Horticultural, Gansu Agricultural University, Lanzhou 730070, China;
2. Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing 100097, China;
3. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2016-03-18 Published:2016-06-28

摘要/Abstract

摘要： 旨在发掘甘蓝抗枯萎病基因FOC1中与抗性相关的特异性单核苷酸多态性(SNP),为进一步开发FOC1特异分子标记提供理论依据。在对11份甘蓝自交系材料枯萎病抗病表型鉴定基础上,通过基因测序,对每份材料中FOC1等位基因及相应的氨基酸序列变异进行分析。结果表明,FOC1等位基因序列之间共存在92处SNP变异和2处Indel变异,其中包含C381A/G等18个在抗、感材料中表现出特异性差异的SNPs。此18个特异的SNP中转换型比率为86.11%、颠换型比率为13.89%,4种碱基变异率以T/C、G/A最高,分别占47.22%和38.89%,而C/G和A/C变异率共占13.89%。本研究也发现抗、感材料中存在4个特异的SNP,可造成3个氨基酸的变化。

关键词: 甘蓝, 抗枯萎病, 特异SNP, 序列分析

Abstract: The aim of this study was to uncover the specific SNPs of fusarium wilt resistance gene FOC1 of Brassica oleracea, which will lay the foundation for the development of specific molecular markers in the future.Based on the identification of fusarium wilt resistance in 11 cabbage inbred lines,the variations of gene sequences and amino acid sequences of FOC1 and its alleles in 11 cabbage materials were analyzed.Results showed that there were 92 SNPs and 2 Indels between these sequences,including 18 specific SNPs related to fusarium wilt resistance,of which 86.11% were transition mutations and 13.89% were transversion mutations.The nucleotide substitutions of T/C (47.22%) and G/A (38.89%) showed higher mutation rates,while C/G and A/C accounting for about 13.89%.In addition,the four specific SNPs identified between the susceptible and resistant cabbage materials led to the variations of three amino acids.

Key words: Brassica oleracea, Fusarium wilt resistance, Specific SNP, Sequence analysis

中图分类号:

雷蕾, 马建, 吕红豪, 颉建明, 郁继华, 康俊根. 甘蓝枯萎病抗性基因FOC1序列分析及抗性相关变异位点分析[J]. 华北农学报, 2016, 31(3): 1-10. doi: 10.7668/hbnxb.2016.03.001.

LEI Lei, MA Jian, LV Honghao, XIE Jianming, YU Jihua, KANG Jungen. Analysis of Sequences and Variation Sites of Fusarium Wilt Resistance Gene FOC1 in Brassica oleracea[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(3): 1-10. doi: 10.7668/hbnxb.2016.03.001.

http://www.hbnxb.net/CN/Y2016/V31/I3/1

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甘蓝枯萎病抗性基因FOC1序列分析及抗性相关变异位点分析

Analysis of Sequences and Variation Sites of Fusarium Wilt Resistance Gene FOC1 in Brassica oleracea

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甘蓝枯萎病抗性基因FOC1序列分析及抗性相关变异位点分析

Analysis of Sequences and Variation Sites of Fusarium Wilt Resistance Gene FOC1 in Brassica oleracea

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