硫硒钨对钼酸盐吸收及钼酸盐转运子的影响

doi:10.7668/hbnxb.2015.05.036

摘要/Abstract

摘要： 为研究硫酸盐、硒酸盐和钨酸盐对甘蓝型油菜钼吸收及钼转运子的影响,通过溶液培养试验,研究了不同竞争离子对甘蓝型油菜生长及钼含量的影响及对预测钼酸盐转运基因表达的影响。结果表明,缺硫处理显著降低单株干物质重和地上部硫含量,同时提高了地上部和地下部钼含量。施硒处理对钼含量影响不大,施钨处理显著降低了钼含量,说明钨对钼吸收具有更高的抑制作用。同时发现,缺钼处理对硫含量没有显著影响。另外,通过测定拟南芥 MOT1 和 MOT2 同源基因在甘蓝型油菜中表达可以看出,相对于 Bol001439 和 Bal035189,基因 Bra007781、 Bol020963 和 Bra011159 整体在地上部和地下部都具有较高的表达水平。 Bra007781、 Bol020963 和 Bra011159 主要在地上部表达。 Bol001439和Bal035189 具有较低的表达,但是缺钼显著影响了根部表达。相对于+Se,+W处理对 BnMOT1s 表达量具有较高的影响,对 BnMOT2s 具有低的影响。推测 Bra007781、Bol001439 是钼高亲和转运子, Bol020963 和 Bra011159 是钼低亲和转运子, Bra035189 不参与钼转运,而 ST5; 1 是一个硫酸盐和钼酸盐共转运子。通过竞争离子对油菜钼营养进行研究且首次对甘蓝型油菜上钼酸盐转运基因进行推测。

关键词: 钼, 同源基因, 硫酸盐, 硒酸盐, 钨酸盐, 甘蓝型油菜

Abstract: Our objective was to explore the effects of sulfur (S),selenium (Se),and tungsten (W) application on growth and molybdenum (Mo) concentrations of oilseed rape and predicted molybdate transporter gene expression were analyzed using hydroponics.The dry weight and shoot S concentration were decreased with S-deficiency,but the Mo concentration was increased slightly.However,the Mo concentration showed no effect with supplied Se,while the Mo level showed lower Mo levels with W treatment.The S concentration was not affect by Mo deficiency.In addition,expression levels of Arabidopsis thaliana MOT1 and MOT2 homologous genes in all treatments were reported and indicate Bra007781, Bol020963,and Bra011159 had a higher expression levels than Bol001439 and Bal035189 in both shoot and root.And the Bra007781, Bol020963,and Bra011159 gene were expressed mainly in shoot. Bol001439 and Bal035189 were poorly expressed except root under Mo-deficient conditions. Bra007781 and Bol001439 were peculated the high-affinity molybdate transporter,and Bol020963 and Bra011159 were peculated the low-affinity molybdate transporter. Bra035189 was not taken into transport process. ST5 ; 1 was speculated a co-transporter in sulfate and molybdate transporter.In conclusion,competing ions (S,Se,and W) application had a marked impact on Mo distribution and accumulation,which was the first investigation of effect of S,Se,and W on Mo transporter homologous genes expression of rapeseed.

Key words: Molybdenum, Homologous genes, Sulfur, Selenium, Tungsten, Brassica napus

中图分类号:

S143.7

秦世玉, 孙学成, 胡承孝, 谭启玲. 硫硒钨对钼酸盐吸收及钼酸盐转运子的影响[J]. 华北农学报, 2015, 30(5): 232-238. doi: 10.7668/hbnxb.2015.05.036.

QIN Shi-yu, SUN Xue-cheng, HU Cheng-xiao, TAN Qi-ling. Sulfur-selenium-tungsten Compete the Molybdate Uptake by Modifying the Gene Expression of Predicted Molybdate Transporters[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(5): 232-238. doi: 10.7668/hbnxb.2015.05.036.

http://www.hbnxb.net/CN/Y2015/V30/I5/232

参考文献

[1] Sigel R K.Molybdenum and tungsten:their roles in biological processes[M].Dekker,2002.
[2] Zhang Y,Gladyshev V N.Molybdoproteomes and evolution of molybdenum utilization[J].Journal of Molecular Biology.2008,379(4):881-899.
[3] Havemeyer A,Bittner F,Wollers S,et al.Identification of the missing component in the mitochondrial benzamidoxime prodrug-converting system as a novel molybdenum enzyme[J].Journal of Biological Chemistry,2006,281(46):34796-34802.
[4] Mendel R R,Kruse T.Cell biology of molybdenum in plants and humans[J].Biochimica et Biophysica Acta(BBA)-Molecular Cell Research,2012,1823(9):1568-1579.
[5] Schwarz G,Mendel R R,Ribbe M W.Molybdenum cofactors,enzymes and pathways[J].Nature,2009,460(7257):839-847.
[6] Hille R.The mononuclear molybdenum enzymes[J].Chemical Reviews,1996,96(7):2757-2816.
[7] Liu H,Hu C,Sun X,et al.Interactive effects of molybdenum and phosphorus fertilizers on photosynthetic characteristics of seedlings and grain yield of Brassica napus[J].Plant and Soil,2010,326(1-2):345-353.
[8] Liu H,Hu C,Sun X,et al.Interactive effects of molybdenum and phosphorus fertilizers on grain yield and quality of Brassica napus[J].J Food Agric Environ,2009,7:266-269.
[9] Kaiser B N,Gridley K L,Brady J N,et al.The role of molybdenum in agricultural plant production[J].Annals of Botany,2005,96(5):745-754.
[10] 张木,胡承孝,刘金山,等.叶面喷施微量元素和氨基酸对不同氮水平小白菜产量及品质的影响[J].长江蔬菜,2011(10):53-58.
[11] Gupta U C.Soil and plant factors affecting molybdenum uptake by plants[J].Molybdenum in Agriculture,1997:71-91.
[12] Reddy K J,Munn L C,Wang L.Chemistry and mineralogy of molybdenum in soils[J].Molybdenum in Agriculture,1997:4-22.
[13] Bolchi A,Petrucco S,Tenca P L,et al.Coordinate modulation of maize sulfate permease and ATP sulfurylase mRNAs in response to variations in sulfur nutritional status:stereospecific down-regulation by L-cysteine[J].Plant Molecular Biology,1999,39(3):527-537.
[14] Buchner P,Takahashi H,Hawkesford M J.Plant sulphate transporters:co-ordination of uptake,intracellular and long-distance transport[J].Journal of Experimental Botany,2004,55(404):1765-1773.
[15] Howarth J R,Fourcroy P,Davidian J,et al.Cloning of two contrasting high-affinity sulfate transporters from tomato induced by low sulfate and infection by the vascular pathogen Verticillium dahliae[J].Planta,2003,218(1):58-64.
[16] Yoshimoto N,Inoue E,Saito K,et al.Phloem-localizing sulfate transporter,Sultr1; 3,mediates re-distribution of sulfur from source to sink organs in Arabidopsis[J].Plant Physiology,2003,131(4):1511-1517.
[17] Yoshimoto N,Takahashi H,Smith F W,et al.Two distinct high-affinity sulfate transporters with different inducibilities mediate uptake of sulfate in Arabidopsis roots[J].The Plant Journal,2002,29(4):465-473.
[18] Fitzpatrick K L,Tyerman S D,Kaiser B N.Molybdate transport through the plant sulfate transporter SHST1[J].FEBS letters,2008,582(10):1508-1513.
[19] Baxter I,Muthukumar B,Park H C,et al.Variation in molybdenum content across broadly distributed populations of Arabidopsis thaliana is controlled by a mitochondrial molybdenum transporter(MOT1)[J].PLoS Genetics,2008,4(2):e1000004.
[20] Tejada-Jiménez M,Llamas Á,Sanz-Luque E,et al.A high-affinity molybdate transporter in eukaryotes[J].Proceedings of the National Academy of Sciences,2007,104(50):20126-20130.
[21] Tomatsu H,Takano J,Takahashi H,et al.An Arabidopsis thaliana high-affinity molybdate transporter required for efficient uptake of molybdate from soil[J].Proceedings of the National Academy of Sciences,2007,104(47):18807-18812.
[22] Tejada-Jiménez M,Galván A,Fernández E.Algae and humans share a molybdate transporter[J].Proceedings of the National Academy of Sciences,2011,108(16):6420-6425.
[23] Gasber A,Klaumann S,Trentmann O,et al.Identification of an Arabidopsis solute carrier critical for intracellular transport and inter-organ allocation of molybdate[Z].2011:13,710-718.
[24] Ellis D R,Salt D E.Plants,selenium and human health[J].Current Opinion in Plant Biology,2003,6(3):273-279.
[25] Shibagaki N,Rose A,Mcdermott J P,et al.Selenate-resistant mutants of Arabidopsis thaliana identify Sultr1; 2,a sulfate transporter required for efficient transport of sulfate into roots[J].The Plant Journal,2002,29(4):475-486.
[26] El Kassis E,Cathala N,Rouached H,et al.Characterization of a selenate-resistant Arabidopsis mutant.Root growth as a potential target for selenate toxicity[J].Plant Physiology,2007,143(3):1231-1241.
[27] Terry N,Zayed A M,De Souza M P,et al.Selenium in higher plants[J].Annual review of plant biology.2000,51(1):401-432.
[28] Sors T G,Ellis D R,Salt D E.Selenium uptake,translocation,assimilation and metabolic fate in plants[J].Photosynthesis Research,2005,86(3):373-389.
[29] Hille R.Molybdenum and tungsten in biology[J].Trends in Biochemical Sciences(Regular Edition),2002,27(7):360-367.
[30] Mendel R R.Biology of the molybdenum cofactor[J].Journal of Experimental Botany,2007,58(9):2289-2296.
[31] Mendel R R.Molybdenum:biological activity and metabolism[J].Dalton Transactions,2005(21):3404-3409.
[32] Mendel R R,Hänsch R.Molybdoenzymes and molybdenum cofactor in plants[J].Journal of Experimental Botany,2002,53(375):1689-1698.
[33] Zimmer W,Mendel R.Molybdenum metabolism in plants[J].Plant Biology,1999,1(2):160-168.
[34] Chan M K,Mukund S,Kletzin A,et al.Structure of a hyperthermophilic tungstopterin enzyme,aldehyde ferredoxin oxidoreductase[J].Science,1995,267(5203):1463-1469.
[35] Foley J A,Ramankutty N,Brauman K A,et al.Solutions for a cultivated planet[J].Nature,2011,478(7369):337-342.
[36] Adamakis I D S,Panteris E,Eleftheriou E P.Tungsten toxicity in plants[J].Plants,2012,1(2):82-99.
[37] Zhang M,Hu C,Zhao X,et al.Impact of molybdenum on Chinese cabbage response to selenium in solution culture[J].Soil Science and Plant Nutrition,2012,58(5):595-603.
[38] Harris J,Schneberg K A,Pilon-Smits E A.Sulfur-selenium-molybdenum interactions distinguish selenium hyperaccumulator Stanleya pinnata from non-hyperaccumulator Brassica juncea(Brassicaceae)[J].Planta,2014,239(2):479-491.
[39] Kumar A,Aery N C.Effect of tungsten on growth,biochemical constituents,molybdenum and tungsten contents in wheat[J].Plant Soil Environ,2011,57(11):519-525.
[40] Shinmachi F,Buchner P,Stroud J L,et al.Influence of sulfur deficiency on the expression of specific sulfate transporters and the distribution of sulfur,selenium,and molybdenum in wheat[J].Plant Physiology,2010,153(1):327-336.
[41] Schiavon M,Pittarello M,Pilon-Smits E,et al.Selenate and molybdate alter sulfate transport and assimilation in Brassica juncea L.Czern.:Implications for phytoremediation[J].Environmental and Experimental Botany,2012,75:41-51.

选择文件类型/文献管理软件名称

选择包含的内容