低温胁迫下丛枝菌根真菌对玉米幼苗形态、生长和光合的影响

doi:10.7668/hbnxb.2014.S1.031

华北农学报 ›› 2014, Vol. 29 ›› Issue (S1): 155-161. doi: 10.7668/hbnxb.2014.S1.031

所属专题： 玉米；

低温胁迫下丛枝菌根真菌对玉米幼苗形态、生长和光合的影响

陈笑莹^1,2, 宋凤斌¹, 朱先灿¹, 孙露莹^1,2, 马福^1,2, 刘胜群¹

1. 中国科学院东北地理与农业生态研究所, 吉林长春 130102;
2. 中国科学院大学, 北京 100049

收稿日期:2014-09-26 出版日期:2014-12-28
通讯作者: 宋凤斌(1963-),男,吉林前郭人,研究员,博士,博士生导师,主要从事作物生理生态研究。
作者简介:陈笑莹(1984-),女,吉林长春人,在读博士,主要从事菌根与植物抗逆关系研究。
基金资助:
国家科技支撑计划项目(2012BAD42B01);国家自然科学基金项目(41301314);中国科学院知识创新项目(KSCX2-YW-N-077);国家自然科学基金项目(31000690)

Effect of Arbuscular Mycorrhizal Fungus on Morphology, Growth and Photosynthetic Characteristics in Maize Seedlings under Low Temperature Stress

CHEN Xiao-ying^1,2, SONG Feng-bin¹, ZHU Xian-can¹, SUN Lu-ying^1,2, MA Fu^1,2, LIU Sheng-qun¹

1. Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2014-09-26 Published:2014-12-28

摘要/Abstract

摘要： 为了解丛枝菌根真菌对玉米抗寒性的作用及其机理,利用盆栽试验,研究了低温胁迫下丛枝菌根(AM)真菌接种处理对玉米幼苗形态、生长和光合特征的影响。研究显示,低温胁迫下,与非AM真菌接种处理玉米相比,接种玉米具有更大的叶片大小和更高的地上生物量。低温胁迫降低了玉米叶片净光合速率(Pn)、气孔导度(Gs)和蒸腾速率(Tr),增加了叶片胞间CO₂浓度(Ci)。接种丛枝菌根真菌的玉米Pn、Gs和Tr高于不接种植株,而Ci低于不接种植株。低温胁迫下,玉米叶片初始荧光(Fo)升高,而可变荧光(Fv)、最大荧光(Fm)、最大光化学效率(Fm/Fv)和潜在光化学效率(Fv/Fo)均下降。无论常温或低温,接种AM真菌玉米叶片Fm和Fv高于非接种植株,同时,接种真菌玉米的叶片Fm/Fv和Fv/Fo也均高于不接种植株。研究结果表明,低温能够对玉米幼苗的生长和光合生理造成严重伤害;低温胁迫下,接种AM真菌能够通过其对玉米植株的保护和促进作用改善玉米的生长,以及对叶片光合系统的保护作用提高玉米的光合能力,增强玉米幼苗的抗寒能力。

关键词: 低温胁迫, 丛枝菌根, 叶绿素荧光, 光合作用

Abstract: For understanding the effects and effect mechanisms of arbuscular mycorhizal(AM) fungus on low temperature tolerance of maize.The effect of arbuscular mycorhizal(AM) fungus inoculation on morphology,growth and photosynthetic characteristics of maize seedlings was studied in pot culture under low temperature stress.Study showed that under low temperature stress,compared with non AM-inoculated maize plants,AM-inoculated maize plants had larger leaf size and higher aboveground biomass.Low temperature stress decreased the photosynthetic rate(Pn),stomatal conductance(Gs) and transpiration rate(Tr) of maize leaves,but increased the intercellular CO₂ concentration(Ci).The Pn,Gs and Tr of AM-inoculated maize leaves were higher than those of non AM-inoculated plants,and the Ci of AM-inoculated maize leaves was lower than that of non AM-inoculated plants.Under low temperature stress,the primary fluorescence(Fo) was increased,while variable fluorescence(Fv),maximal fluorescence(Fm),maximum photochemical efficiency(Fv/Fm) and potential photochemical efficiency(Fv/Fo) were decreased.Without regard of temperature,compared with AM-inoculated maize plants,AM-inoculated plants had higher Fv,Fm,Fv/Fm and Fv/Fo.The results indicated that low temperature could induce serious damage of growth and photosynthetic physiology of maize seedling;AM inoculation could enhance low temperature tolerance of maize seedling by protective and facilitated effects on maize plants to promote its growth,and protective effect on photosynthetic system to improve its photosynthetic capacity.

Key words: Low temperature stress, Arbuscular mycorrhiza, Chlorophyll fluorescence, Photosynthesis

中图分类号:

S513.01

陈笑莹, 宋凤斌, 朱先灿, 孙露莹, 马福, 刘胜群. 低温胁迫下丛枝菌根真菌对玉米幼苗形态、生长和光合的影响[J]. 华北农学报, 2014, 29(S1): 155-161. doi: 10.7668/hbnxb.2014.S1.031.

CHEN Xiao-ying, SONG Feng-bin, ZHU Xian-can, SUN Lu-ying, MA Fu, LIU Sheng-qun. Effect of Arbuscular Mycorrhizal Fungus on Morphology, Growth and Photosynthetic Characteristics in Maize Seedlings under Low Temperature Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(S1): 155-161. doi: 10.7668/hbnxb.2014.S1.031.

http://www.hbnxb.net/CN/Y2014/V29/IS1/155

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