摘要： 为了研究FT/TFL类基因山葡萄雌花和雄花中的表达模式,以山葡萄3个雄花品系和3个雌花品系为材料,根据GenBank上已经发表的3个葡萄FT/TFL类基因设计引物,利用实时荧光定量PCR技术研究VvFT、VvTFL1A 和VvTFL1B 这3个基因在山葡萄雌花和雄花中的表达。3个基因在山葡萄雌花和雄花中都有表达。VvFT基因在雌花中的表达量显著高于雄花。而VvTFL1A 和VvTFL1B 这2个基因在雄花中的表达量略高于雌花。VvFT基因的表达高峰出现在花序刚开始展露期(5月14日);而VvTFL1A 和VvTFL1B 表达高峰出现在芽体膨大期(4月30日)。结果表明,这两类基因在山葡萄花发育过程中作用时间不同。FT 类基因可能参与花的前期调控,而TFL 类基因在花器官发育中起作用。

关键词: 山葡萄, 实时荧光定量PCR, FT/TFL基因

Abstract: To study FT/TFL genes expression pattern in female and male floral of V itis amurensis Rupr.,three male and three female strains were used to study the expression of three FT/TFL genes ( VvFT,VvTFL1A and VvTFL1B) by Real-time PCR technology.The three genes expressed not only in female but also in male floral.The relative expression of VvFT gene in female were significantly higher than in male floral,while the relative expression of VvTFL1A and VvTFL1B genes in male flowers slightly higher than in female floral.The peak value of VvFT gene expression appeared at inflorescence show stage (May 14);while VvTFL1A and VvTFL1B expression appeared one peak value at bud intumescence stage (April 30).It was suggested that FT genes may be involved in regulation of early flowers,and TFL genes played a role in floral organ development.

Key words: Vitis amurensis Rupr., Real-time PCR, FT/TFL gene

中图分类号: 

• Q78

• S663.1