摘要： 从灰葡萄孢ATMT突变体库中筛选获得一株不产生菌核的突变体,并利用TAIL-PCR技术获得插入位点的侧翼序列,将其在灰葡萄孢基因组数据库中进行Blast比对分析,采用分子生物学技术,对突变菌株BMH174鉴定并获得其突变基因（BC1G₀6945.1）;并对突变体的形态学、生长速率、分生孢子产量、致病能力、胞壁降解酶活力、毒素的生物学活性等方面进行了研究,这将为深入了解灰葡萄孢的致病机制奠定基础。

关键词: 灰葡萄孢, T-DNA插入突变体, TAIL-PCR

Abstract: One sclerotia-defect mutant of Botrytis cinerea BMH174 was obtained by Agrobacterium tumefaciensmediated method. The flanking sequence was acquired by TAIL-PCR, and then the T-DNA insertion gene confirmed by Blast in the B. cinerea gene database. The mutant gene was identified BC1G_06945. 1 gene by molecular biolo- gy. The sclerotia formation and pathogenicity in B. cinerea which will facilitate to understand molecular mechanism of conidium development, sclerotia formation and pathogenic in B. cinerea.

Key words: Botrytis cinerea, T-DNA insertional mutant, TAIL-PCR

中图分类号: 

• S436.631