摘要： 为建立黄瓜抗黑斑病分子标记辅助育种体系,以黄瓜感黑斑病母本L 63和抗黑斑病父本L 9及其F1、F，苏北》2,/sub>分离群体为试材,将与黑斑病抗性相关基因连锁的一个共显性AFLP标记E-CC/M-CAT进行了测序,根据序列特点设计了特异的SCAR引物SCEM126/122,成功地转换成了简单实用的共显性SCAR标记,经验证该标记与黄瓜抗黑斑病相关基因连锁遗传距离为4.4 cM,可以作为黄瓜抗黑斑病辅助选择的标记。且该标记具有迅速、简便、成本低、不受环境条件限制的优点,扩增条带清晰,无杂带和拖尾现象,适合用于大量样本分析。利用引物SCEM126/122对290份材料进行抗病性检测,结果表明有64份抗病材料,为进行抗黑斑病黄瓜新品种的选育奠定了基础。
关键词:
黄瓜,
黑斑病,
SCAR,
资源
Abstract: In order to establish molecular marker-assisted selection system of cucumber alternaria leaf spot with F 1,F 2 and BC 1 population between a susceptible parent(L 63) and a resistant parent(L 9),a co-dominant AFLP marker was successfully converted into a simple,applied and co-dominant SCAR marker. Special marker SCEM 126 /122 were designed according to the sequence information of AFLP(E-CC / M-CAT) fragments. The marker was closely linked to the cucumber alternaria leaf spot resistance-related gene,and the genetic distance between the marker and the gene was 4. 4 cM. The marker could be useful in marker-assisted selection in cucumber breeding. The acquired SCAR marker had many advantages including fast,accurate,low cost,free from an environmental influence et. 64 resistant germplasm were abtained from total 290. Application of the acquired marker will inhance cucumber alternaria leaf spot disease resistant germplasm evaluation.
Key words:
Cucumber,
Alteruaria leaf spot,
SCAR,
Germplasm
中图分类号:
王惠哲, 李淑菊, 杨瑞环, 管炜, 邓强, 曹明明. 与黄瓜抗黑斑病基因连锁的SCAR标记及抗病资源筛选[J]. 华北农学报, 2014, 29(1): 208-212. doi: 10.7668/hbnxb.2014.01.037.
WANG Hui-zhe, LI Shu-ju, YANG Rui-huan, GUAN Wei, DENG Qiang, CAO Ming-ming. SCAR Marker Resistance-related to Cucumber Alternaria Leaf Spot and Resistance Identification of Cucumber Germplasm[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(1): 208-212. doi: 10.7668/hbnxb.2014.01.037.