摘要： 本研究旨在观察饲料中添加不同水平的维生素D3(Vitamin D3)对松浦镜鲤(C.carpio Songpu mirror carp)幼鱼体成分、血清钙磷和碱性磷酸酶(ALP)活性的影响,从而确定鲤鱼幼鱼饲料中维生素D3的最适添加量。选用2.4 g左右的镜鲤幼鱼525尾,随机分为7组,每组设3个重复,每个重复25尾鱼,分别投喂维生素D3添加量为0,200,400,800,1 600,3 200及1×105IU/kg的7种饲料,饲养试验时间为56 d。结果表明:饲料中添加维生素D3对镜鲤鱼体水分、粗蛋白、粗脂肪含量和血清磷离子浓度没有显著影响(P>0.05)。与未添加维生素D3的试验组相比,饲料中添加维生素D3对镜鲤鱼体灰分、钙磷含量、血清中钙离子浓度和碱性磷酸酶活性影响显著(P<0.05)。随着维生素D3添加量的增加,全鱼钙磷含量、血清钙磷离子浓度和碱性磷酸酶活性均有先升高后降低的趋势。结果提示:与饲料中缺乏或过量的维生素D3添加量相比,适量添加维生素D3可使镜鲤全鱼钙磷含量、血清中钙离子浓度和碱性磷酸酶活性显著升高(P<0.05)。以全鱼钙磷含量和血清中碱性磷酸酶活性为指标建立折线模型进行计算,得出松浦镜鲤幼鱼饲料中维生素D3最适添加量为708.3～769.8 IU/kg。
关键词:
维生素D3,
镜鲤,
体成分,
碱性磷酸酶
Abstract: This experiment was conducted to study the effect of vitamin D3 levels on body composition,concentration of calcium and phosphorus and activity of alkaline phosphatase(ALP) in the serum of juvenile mirror carp(C.carpio Songpu mirror carp.),to determine the best dietary vitamin D3 level of juvenile carp.525 juvenile mirror carps,weighting an average of 2.4 g were randomly assigned to seven dietary treatments with three replicates and 25 carps with each pen.Seven levels of vitamin D3(0,200,400,800,1 600,3 200 and 1×105 IU/kg) of the dietary were fed to seven groups.The feeding trial lasted for 56 days.The results showed that moisture,crude protein,crude lipid contents of the body and concentration of phosphorus in the serum were not significantly affected by vitamin D3(P0.05).The crude ash,calcium and phosphorus contents of the body,concentration of calcium and activity of ALP in the serum were significantly affected by vitamin D3(P0.05),compared to the experimental group which without vitamin D3.Calcium and phosphorus contents of the body,concentration of calcium and phosphorus and activity of ALP in the serum were increased and then decreased with increasing of dietary vitamin D3 supplementation.These results indicated that the calcium and phosphorus contents of the body,concentration of calcium and activity of ALP in the serum was significantly increased by suitable vitamin D3 level,compared with no or excessive vitamin D3 level.The suitable requirement of juvenile mirror carp is about 708.3-769.8 IU/kg diet,based on establishing indexes line model to calculate the calcium,phosphorus contents of the body and ALP activity of mirror carp in the serum.
Key words:
Vitamin,
D3,
Mirror carp,
Body composition,
ALP
中图分类号:
张桐, 徐奇友, 许红, 王常安, 石连玉. 饲料中维生素D3对松浦镜鲤幼鱼体成分和血清碱性磷酸酶的影响[J]. 华北农学报, 2011, 26(S1): 258-263. doi: 10.7668/hbnxb.2011.S1.052.
ZHANG Tong, XU Qi-you, XU Hong, WANG Chang-an, SHI Lian-yu. Effects of Dietary Vitamin D_3 Supplementation on Body Composition and Activity of Alkaline Phosphatase in the Serum of Juvenile Mirror Carp(C. carpio Songpu mirror carp)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(S1): 258-263. doi: 10.7668/hbnxb.2011.S1.052.