摘要： 为探讨西芹种子浸提液灌根处理后黄瓜产生抗枯萎病的生理机制,采用蒸馏水、乙醇、丙酮作为浸提剂,获得西芹种子的浸提液,在黄瓜第一真叶横宽5 cm时进行两次灌根处理,然后于不同时期对其叶片内丙二醛含量、电导率、可溶性糖含量、可溶性蛋白质含量进行测定。结果表明,在各取样时期丙二醛含量、电导率较对照降低,丙二醛含量降低的范围为蒸馏水处理8.65%~23.89%、乙醇处理0.68%~13.89%、丙酮处理7.50%~21.69%;电导率降低的范围为蒸馏水处理0.62%~9.47%、乙醇处理0.40%~11.35%、丙酮处理1.86%~19.80%。可溶性糖、可溶性蛋白质含量在各取样时期均较对照升高,可溶性糖含量升高的范围为蒸馏水处理12.21%~41.50%、乙醇处理6.28%~62.25%、丙酮处理23.43%~54.38%;可溶性蛋白质含量升高的范围为蒸馏水处理0.08%~25.87%、乙醇处理0.93%~23.08%、丙酮处理2.20%~37.67%。各指标的变化幅度均是丙酮处理大于乙醇处理,乙醇处理大于蒸馏水处理。

关键词: 西芹种子浸提液, 黄瓜枯萎病菌, 化感作用机理, 黄瓜叶片, 生理指标

Abstract: The cucumber seeding were irrigated with parasley seed extracts in order to investigated the physiological mechanism for resistance of Cucumber Fusarinm Wilt.the extracts obtained from the parasley seed with distilled water, ethanol and acetone as the extractant.Cucumber seedlings were irrigated(twice time)with the three kinds of the extracts at the stage of 5 cm width of the first true 1eaf.Than the content of MDA.Electrolyte Leakage Rate.the con. tent of soluble sugar and the content of soluble protein in the cucumber lear at difierent times were determined.The results showed that the content of MDA, Electrolyte Leakage Rate was lower than that of the control at the different time.The content of MDA in the cucumber leat"were lower than the control from 8.65%to 23.89%, 0.68%to 13.89%。7.50%to 21.69%irrigated with distilled water extracts, ethanol extracts and acetone extracts, Electrolyte Leakage Rate were lower than the control from 0.62%to 9.47%。0.40%to 11.35%.1.86%to 19.80%irrigated with distilled water extracts.ethan01 extracts and acetone extracts.The content of soluble sugar。soluble protein were higher than that of the control at the different time.The content of soluble sugar were higher than that of the control from 12.21%to 41.50%irrigated with distilled water extracts：6.28%to 62.25%deal with ethanol extracts；23.43% to 54.38%irrigated with acetone extracts；The content of soluble protein were higher than the control from 0.08%to 25.87%irrigated with distilled water extracts；0.93%to 23.08%irrigated with ethanol extracts；2.20%to 37.67% irrigated with acetone extracts；The variation of acetone extracts treatment was the greater than ethanol extracts, ethanol extracts treatment than distilled water extracts.

Key words: xtracts of parsley seed, Fusarium oxysporium f sp.cucumeris, Allelopathy mechanism, Cucumber leaf, Physiology changes

中图分类号: 

• S436.421