冬瓜枯萎病菌核糖体rDNA ITS区的克隆与序列分析

doi:10.7668/hbnxb.2010.01.015

华北农学报 ›› 2010, Vol. 25 ›› Issue (1): 80-83. doi: 10.7668/hbnxb.2010.01.015

所属专题： 西瓜；

冬瓜枯萎病菌核糖体rDNA ITS区的克隆与序列分析

王翠霞, 徐金茹, 习雨琳, 白雪菲, 温晓蕾, 段会军

河北农业大学,河北省作物种质资源重点实验室, 河北保定 071001

收稿日期:2009-08-21 出版日期:2010-02-28
通讯作者: 段会军(1965-),男,河北保定人,教授,博士,主要从事瓜类抗病育种研究.
作者简介:王翠霞(1984-),女,河北邯郸人,硕士,主要从事瓜类抗病育种研究.
基金资助:
河北省自然科学基金资助项目(C2007000547)

Cloning and Sequencing of rDNA ITS Regions in Fusarium oxysporum f.sp.benincasae

WANG Cui-xia,XU Jin-ru,XI Yu-lin,BAI Xue-fei,WEN Xiao-lie,DUAN Hui-jun

Agricultural University of Hebei, Key Laboratory of Crop Germplasm Resources of Hebei, Baoding 071001, China

Received:2009-08-21 Published:2010-02-28

摘要/Abstract

摘要： 采用镰刀菌核糖体基因转录间隔区(ITS)通用引物,PCR扩增冬瓜枯萎病菌核糖体基因ITS区,并对产物进行克隆和序列分析;利用Mega.1软件对序列及GeneBank中以葫芦科为寄主的镰刀菌不同专化型ITS序列进行聚类.冬瓜枯萎病菌ITS全长1 063 bp,其中包括18S rDNA一部分序列,5.8S rDNA,ITS1和ITS2全部序列及28SrDNA部分序列.聚类结果将15个菌株ITS序列划分为2个类群,类群I包括个菌株,分别为2个西瓜枯萎病菌株和2个甜瓜枯萎病菌株;类群II包括11个菌株,其中冬瓜枯萎病菌株就在该类群中,其余为甜瓜枯萎病菌株5个、西瓜枯萎病菌株3个、黄瓜枯萎病菌株、丝瓜枯萎病菌株和葫芦枯萎病菌株各1个.

关键词: 冬瓜, 冬瓜枯萎病菌, ITS

Abstract: The internal transcribed spacer regions (ITS) of the rDNA from Fusarium oxysporum f.sp.Benincasae were amplified with the polymerase chain reaction through the universal primers and sequenced,meanwhile the ITS of different formae specialis of Fusarium oxysporum for the host to Cucurbitaceae were clustered.The total length of ITS in Fusarium oxysporum f.sp.benincasae was 1 063 bp,including partial 18S rDNA,5.8S rDNA,ITS1,ITS2 and partial 28S rDNA.Cluster results showed that 15 isolates were classified into two groups.Group I included 2 Fusarium oxysporum f.sp.niveum and 2 Fusarium oxysporum f.sp.melonis.Group I included 11 isolates,among them Fusarium oxysporum f.sp. benincasae belonged to the group,the others were 5 Fusarium oxysporum f.sp.melonis,3 Fusarium oxysporum f.sp.niveum,1 Fusarium oxysporum f.p.cucumerinum,1 Fusarium oxysporum f.sp.luffae and 1 Fusarium oxysporum f.sp.Lagenariae,respectively.

Key words: Fatmelon, Fusarium oxysporum f.sp.benincasae, Internal transcribed spacer regions

中图分类号:

S436.5

王翠霞, 徐金茹, 习雨琳, 白雪菲, 温晓蕾, 段会军. 冬瓜枯萎病菌核糖体rDNA ITS区的克隆与序列分析[J]. 华北农学报, 2010, 25(1): 80-83. doi: 10.7668/hbnxb.2010.01.015.

WANG Cui-xia,XU Jin-ru,XI Yu-lin,BAI Xue-fei,WEN Xiao-lie,DUAN Hui-jun. Cloning and Sequencing of rDNA ITS Regions in Fusarium oxysporum f.sp.benincasae[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(1): 80-83. doi: 10.7668/hbnxb.2010.01.015.

http://www.hbnxb.net/CN/Y2010/V25/I1/80

参考文献

[1] Woo S L,Zoina A,Del Sorbo G,et al.Characterization of Fusarium oxysprum f.sp.phaseoli by pathogenic races,VCGs,and RAPD[J].Phytopathology,1996,86 (9):966-973.
[2] Wyss P,Bontante P.Aplification of genomic DNA of arbuscular mycrrhizal(AM)fungi by PCR using short arbitrary primers[J].Mycol Res,1993(97):1351-1357.
[3] Tooley P W,O′Neill N R,Goley E D,et al.Assessment of diversity in Claviceps africana and other Claviceps species by RAM and AFLP analyses[J].Phytopathology,2000,90 (10):1126-1130.
[4] Bao J R,Fravel D R,O′-Neill N R,et al.Genetic analysis of pathogenic and nonpathogenic Fusarium oxysporum from tomato plants[J].Canadian Journal of Botany,2002,80(3):271-279.
[5] Manicom B Q,Baayen R P.Restraction fragment length polymorphisms in Fusarium oxysporum f.sp.dianthi and other Fusaria from dianthus species[J].Plant Pathology,1993(42):851-857.
[6] Okoli C A N,Carder J H,Barbara D J.Restriction fragment length polymorphisms (RFLPs) and there relationships of some host-adapted isolates of Verticillium dahliae[J].Plant Pathology,1994,(43):33-40.
[7] Aanen D K,Kuyper T W,Hoekstra R F.A wldely distributed ITS polymorphism within a biological species of the extomycorrhizal fungus Hebeloma velutipes[J].Mycol Res,2001,105(3):284-290.
[8] Amicucci A,Zambonelli A,Giomaro G.Identification of ectomycorrhizal fungi of the genus tuber[J].Mol Ecol,1998,7(3):273-277.
[9] Cooke D E L,Williams N A,Duncan J M.The uses of ITS regions in Phytophthora species[C].Annual Report of the Scottish Crop Research Institute for 1996.Dudee:Scottish Crop Research Institute,1997:151-154.
[10] Cooke D E L,Duncan J M,Drentha,et al.Amolecular phylogeny of phytophthora and related oomycetos[J].Fungal Genetics and Biology,2000,30:17-32.
[11] Kropp B R,Hansenda,Wolfpg,et al.A study on the phylogeny of the Dyer′s woad rust fungus and other species of Puccinia from crucifers[J].Phy-topathology,1997,87:565-571.
[12] Laurene L,Lisa A C,Lori M C,et al.Internet transcribed spacer sequence based phylogeny and polymerase chain reactiom restriction fragment length polymorphism differentiation of tilltia walkeri and T[J].Indica Phytopathology,2001,91:935-940.
[13] Collopypd,Largeteau-mamounml,Romainecpetal.Molecular phylogenetic analyses of Verticilium fungicola and related species causing dry bubble disease of the cultivated button mushroom,Agaricus bisporus[J].Phy topathology,2001,91:905-912.

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冬瓜枯萎病菌核糖体rDNA ITS区的克隆与序列分析

Cloning and Sequencing of rDNA ITS Regions in Fusarium oxysporum f.sp.benincasae

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