基于5.8SrDNA/ITS序列的几种内蒙古棘豆属植物分子系统学研究

doi:10.7668/hbnxb.2009.06.034

华北农学报 ›› 2009, Vol. 24 ›› Issue (6): 168-173. doi: 10.7668/hbnxb.2009.06.034

基于5.8SrDNA/ITS序列的几种内蒙古棘豆属植物分子系统学研究

高静, 卢萍, 王金妞, 金凤, 恩和巴雅尔

内蒙古师范大学, 生命科学与技术学院,内蒙古呼和浩特 010022

收稿日期:2009-10-07 出版日期:2009-12-28
通讯作者: 卢萍(1964-),女,内蒙古海拉尔人,副教授,博士,硕士生导师,主要从事遗传与分子生物学、分子生态学等领域的研究。
作者简介:高静(1984-),女,内蒙古包头人,硕士,主要从事植物分子系统学研究.
基金资助:
内蒙古自然科学基金(200607010504);内蒙古师范大学自然科学基金(QN005021)

Molecular Phylogeny of Several Species of Oxytropis DC.Based on 5.8SrDNA/ITS Sequence

GAO Jing, LU Ping, WANG Jin-niu, JIN Feng, ENHE Bayaer

College of Life Science and Technology, Inner Mongolia Normal University, Huhhot 010022, China

Received:2009-10-07 Published:2009-12-28

摘要/Abstract

摘要： 提取小花棘豆(Oxytropis.glabra DC.)、小叶小花棘豆(O.glabra var.tenuis)、刺叶柄棘豆(O.aciphylla Ledeb.)、砂珍棘豆(O.racemusa Turcz.)、多叶棘豆(O.verticillaris (L.)J.N)、黄毛棘豆(O.ochrantha Turcz.)基因组DNA,扩增其5.8SrDNA/ITS序列并测序,将所测序列与GenBank中疏毛棘豆(O.pilosa)和长白棘豆(O.anertii)5.8SrDNA/ITS序列进行同源性比对,利用MEGA4.0软件采用Neighbor-joining法构建系统发育树,旨在为棘豆属下种间及种下分类研究提供分子依据.结果表明:多叶棘豆、砂珍棘豆和黄毛棘豆构成姐妹群,并在部分地理种群上混杂,认为将三者归入真棘豆亚属Subgen.Euoxytropis轮叶棘豆组Sect.Baicalia Stell.ex Bunge更合理.小叶小花棘豆与小花棘豆亲缘关系很近,支持将其视为变种的观点.刺叶柄棘豆的不同地理种群形成两大分支,对其在亚属水平上的分类还需进一步商榷.研究赞同棘豆属植物为单系起源,认为种内不同地理种群间的亲缘关系在一定程度上受到了植被类型和生境的影响.

关键词: 棘豆属, 5.8SrDNA/ITS, 系统发生

Abstract: Genomic DNAs of Oxytropis glabra,O.glabra var.tenuis,O.aciphylla,O.racemosa,O.verticillaris and O.ochrantha were extracted,then the 5.8SrDNA/ ITS sequences were amplified and sequenced.Homologous of the sequenceswere compared with that of O.pilosa and O.anertii submitted to GenBank.The phylogenetic tree was constructed by NJ methods inMEGA 4.0 software,the purpose of the researchwas to provide molecular evidences for taxonomy of interspecies and intraspecies of Oxytropis DC.The results showed that O.verticillaris,O.ochrantha and O.racemosa were sister groups and some geographical populations of them were mixed.Therefore,it was reasonable to put the three species into Subgen.Euoxytropis Sect.Baicalia Stell.ex Bunge.O.glabra var.tenuis had a close relationship with O.glabra,which supported the opinion of treating O.glabra var.tenuis as a variant of O.glabra.Different populations of O.aciphylla were divided into two major branches,and the subject position of O.aciphylla at subgenus level needs further investigation.The results also supported that Oxytropis DC.was a monophyletic group.It was indicated that the re lationship among different geographic populations within species were influenced by vegetation type and habitat to some extent.

Key words: Oxytropis DC., 5. 8SrDNA/ ITS, Phylogenetic

中图分类号:

Q949.751.9

高静, 卢萍, 王金妞, 金凤, 恩和巴雅尔. 基于5.8SrDNA/ITS序列的几种内蒙古棘豆属植物分子系统学研究[J]. 华北农学报, 2009, 24(6): 168-173. doi: 10.7668/hbnxb.2009.06.034.

GAO Jing, LU Ping, WANG Jin-niu, JIN Feng, ENHE Bayaer. Molecular Phylogeny of Several Species of Oxytropis DC.Based on 5.8SrDNA/ITS Sequence[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(6): 168-173. doi: 10.7668/hbnxb.2009.06.034.

http://www.hbnxb.net/CN/Y2009/V24/I6/168

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